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Chapter 4 The Interrupted Gene

4.1 Introduction . interrupted gene ? A gene in which the coding sequence is not continuous due to the presence of introns.primary (RNA) transcript ? The original unmodified RNA product corresponding to a transcription unit.RNA splicing ? The process of excising introns from RNA and connecting the exons into a continuous mRNA. .

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Chapter 4 The Interrupted Gene

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    1. Chapter 4 The Interrupted Gene

    2. 4.1 Introduction interrupted gene A gene in which the coding sequence is not continuous due to the presence of introns. primary (RNA) transcript The original unmodified RNA product corresponding to a transcription unit. RNA splicing The process of excising introns from RNA and connecting the exons into a continuous mRNA.

    3. 4.1 Introduction intron A segment of DNA that is transcribed, but later removed from within the transcript by splicing together the sequences (exons) on either side of it. mature transcript A modified RNA transcript. Modification may include the removal of intron sequences and alterations to the 5' and 3' ends.

    5. 4.2 An Interrupted Gene Consists of Exons and Introns Introns are removed by RNA splicing, which occurs in cis in individual RNA molecules. Mutations in exons can affect polypeptide sequence; mutations in introns can affect RNA processing and hence may influence the sequence and/or production of a polypeptide.

    6. 4.3 Exon and Intron Base Compositions Differ The four rules for DNA base composition are the first and second parity rules, the cluster rule, and the GC rule. The second parity rule suggests an extrusion of structured stem-loop segments from duplex DNA, which would be greater in introns. The rules relate to genomic characteristics, or pressures, that constitute the genome phenotype.

    7. 4.4 Organization of Interrupted Genes May Be Conserved Introns can be detected when genes are compared with their RNA transcription products by either restriction mapping, electron microscopy, or sequencing. cDNA A single-stranded DNA complementary to an RNA, synthesized from it by reverse transcription in vitro.

    8. 4.4 Organization of Interrupted Genes May Be Conserved The positions of introns are usually conserved when homologous genes are compared between different organisms. The lengths of the corresponding introns may vary greatly, though. Introns usually do not encode proteins.

    10. 4.5 Exon Sequences under Negative Selection Are Conserved but Introns Vary Comparisons of related genes in different species show that the sequences of the corresponding exons are usually conserved, but the sequences of the introns much less so, when the exons are under selective pressure to retain the capacity to encode useful proteins.

    11. 4.6 Exon Sequences under Positive Selection Vary but Introns Are Conserved Under positive selection an individual with an advantageous mutation is able to produce more progeny than others without the mutation. Due to intrinsic genomic pressures, such as that which conserves the potential to extrude stem-loops from duplex DNA, introns evolve more slowly than exons that are under positive selection pressure.

    13. 4.7 Genes Show a Wide Distribution of Sizes Most genes are uninterrupted in S. cerevisiae, but are interrupted in multicellular eukaryotes.

    14. 4.7 Genes Show a Wide Distribution of Sizes Exons are usually short, typically coding for <100 amino acids.

    15. 4.7 Genes Show a Wide Distribution of Sizes Introns are short in unicellular/oligocellular eukaryotes, but can be many kb in multicellular eukaryotes. The overall length of a gene is determined largely by its introns.

    16. 4.8 Some DNA Sequences Code for More Than One Polypeptide Usage of alternative translation initiation or termination codons allows one polypeptide to be equivalent to a fragment of another. overlapping gene A gene in which part of the sequence is found within part of the sequence of another gene.

    17. 4.8 Some DNA Sequences Code for More Than One Polypeptide Different polypeptides can be produced from the same sequence of DNA when the mRNA is read in different reading frames (as two overlapping genes).

    18. 4.8 Some DNA Sequences Code for More Than One Polypeptide Otherwise identical polypeptides, differing by the presence or absence of certain regions, can be generated by alternative splicing when certain exons are included or excluded. This may take the form of including or excluding individual exons, or of choosing between alternative exons.

    20. 4.9 Some Exons Can Be Equated with Protein Functional Domains Proteins can consist of independent functional modules the boundaries of which, in some cases, can be equated with those of exons.

    21. 4.9 Some Exons Can Be Equated with Protein Functional Domains The exons of some genes appear homologous to the exons of others, suggesting a common exon ancestry.

    22. 4.10 Members of a Gene Family Have a Common Organization gene family A set of genes within a genome that codes for related or identical proteins or RNAs. The members were derived by duplication of an ancestral gene followed by accumulation of changes in sequence between the copies. Most often the members are related but not identical.

    23. 4.10 Members of a Gene Family Have a Common Organization superfamily A set of genes all related by presumed descent from a common ancestor, but now showing considerable variation. A set of homologous genes (homologs) should share common features that preceded their evolutionary separation.

    24. 4.10 Members of a Gene Family Have a Common Organization All globin genes have a common form of organization with three exons and two introns, suggesting that they descended from a single ancestral gene.

    25. 4.10 Members of a Gene Family Have a Common Organization Intron positions in the actin gene family are highly variable, which suggests that introns do not separate functional domains.

    26. 4.11 Genetic Information Is Not Completely Contained in DNA Genetic information includes not only that related to characters corresponding to the conventional phenotype, but also that related to characters (pressures) corresponding to the genome phenotype. In certain contexts, the definition of the gene can be seen as reversed from one gene : one protein to one protein : one gene. Positional information may be important in development.

    27. 4.11 Genetic Information Is Not Completely Contained in DNA Sequences transferred horizontally from other species to the germline could locate within introns or intergenic DNA and thence transfer vertically through the generations. Some of these may be involved in intracellular nonself recognition.

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