1 / 19

RESEARCH REPORT

RESEARCH REPORT. PRESENTED BY DR. M.A. OYEKUNLE DEPARTMENT OF VETERINARY MICROBIOLOGY & PARASITOLOGY UNAAB. IRG 17. SOURCES AND DISTRIBUTION OF SALMONELLA SEROTYPES IN SOME SELECTED FOODS IN SOUTHWESTERN NIGERIA. BY M.A . Oyekunle , M. Agbaje , G.R. Afolabi ,

pomona
Download Presentation

RESEARCH REPORT

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. RESEARCH REPORT PRESENTED BY DR. M.A. OYEKUNLE DEPARTMENT OF VETERINARY MICROBIOLOGY & PARASITOLOGY UNAAB

  2. IRG 17. SOURCES AND DISTRIBUTION OF SALMONELLA SEROTYPES IN SOME SELECTED FOODS IN SOUTHWESTERN NIGERIA BY M.A. Oyekunle, M. Agbaje, G.R. Afolabi, O.O. Kehunde, O.T Kehinde, O.P. Sobukiola, R.M. Dansu, and A. Ricci.

  3. INTRODUCTION • Salmonella are Gram-negative enteric bacteria comprising of two species namely Salmonella enteric and Salmonella bongori. • The latter specie is rarely isolated from human infections. • The Salmonella enteric (S. enteric) which is pathogenic to man and animals is subdivided into subspecies namely S. enteric subsp. entrica. • Salmonellaeare divided into several serotypes based on the differences in their somatic, flagella and capsular antigens. • They are referred to as serotypes because they are identified using sera containing antibody against known strains of Salmonella

  4. INTRODUCTION (CONTD) • The disease caused bySalmonella is Salmonellosis • It can be contracted from many animal sources, vegetables and fruits. • Food animals serve as reservoirs for Salmonella pathogens without necessarily manifesting clinical disease. • Most plant food get contaminated by Salmonellae either through the use of manure or directly the animals while browsing in search of food.

  5. OBJECTIVE OF STUDY • To identify the food sources and distribution of Salmonella serotypes in parts of Ogun, Lagos and Oyo States of Nigeria - Such information will be of immense importance to researcher, public health practitioners and policy makers

  6. MATERIALS AND METHODS Sample collection Food samples comprising: • Beef • Chicken • Fish • Eggs • Local cheese (Wara) • Fermented milk (Nunu) • Some fruits • Vegetables • some food ingredients were randomly purchased in triplicates ( where possible) from markets inAbeokuta, Sagamu, ijebu-ode, Lagos (mile 12) and Ibadan (Bodija).

  7. ISOLATION OF SALMONELLA FROM SAMPLES Sterile water rinse of solid samples and liquid sampleswere cultured as follows: • Pre-enrichment in buffered peptone water for 24-hours at 370C • Selective enrichment of the pre-enriched samples in tetrathionatebroth for 24-hours at 370C • Sub- cultivation of the selectively enriched samples in Salmonella Chromogenic medium for 24-48 hours 370C.

  8. IDENTIFICATION OF THE ISOLATES • Isolates resembling Salmonella on Chromogenic medium were further examined biochemically and by slide agglutination test using Salmonella polyvalent antisera.

  9. SEROTYPING OF ISOLATES • All isolates identified to be Salmonellaare to be serotyped at the Office of International Epizootics (OIE) SalmonellaReference Laboratory in Padova, Italy by Dr. Ricci, A, a co-investigator in this work.

  10. RESULTS

  11. RESULTS (CONTD)

  12. RESULTS (CONTD)

  13. DISCUSSION • A total of One hundred and fifty-nine (159) food items were sampled. • Fifty three (53) from Abeokuta • Thirty (30) from Sagamu • Thirty (30) from Ijebu-ode • Twenty (20) from Lagos and • Nineteen (19) from Ibadan.

  14. DISCUSSION (CONTD) • Out of these, a total of 19 ( 11.9%) were positive for Salmonella arising from • 13 (24.5%) from Abeokuta, • 4 (13.3%) from Sagamu, • 2 (6.67%) from Ijebu-ode and • none from Lagos and Ibadan. • Among the food of animal origin, fish was the highest (25%) carrier followed by beef (20%) and Chicken (12.5%). Food items that are eaten raw and found to carry Salmonella are Carrot (20%), Cucumber (11%), Cabbage (12.5%), Apple (10%), Garden-egg (20%) and Tomatoes(13.3%).

  15. DISCUSSION (CONTD) • Many animals including cattle, fowl and rodents are naturally infected with a variety of Salmonella and have the bacteria in their tissue (meat), excreta or eggs. • The reason for high carrier rate of Salmonella by fish will need further investigation, but could be due to the practice of feeding dead chicken to fish in this part of the country.

  16. DISCUSSION (CONTD) • Inadequate cooking of food can result in food borne infection of Salmonella. • Cross-contamination of other foods can result in the transmission of Salmonella infection to human. • The question of whether there is a common origin of Salmonella inOgun State will become clearer when the result of the serotyping of the isolates in Italy is available.

  17. RECOMMENDATIONS • Thorough cooking of all food stuffs derived from animal sources particularly fish, chicken and beef. • Recontamination within the kitchen after cooking is completed should be avoided • All food to be taken raw should be thoroughly washed before consumption

  18. RECOMMENDATIONS (CONTD) • Education of food handlers and housewives on the necessity of washing their hands before and after food preparation, maintaining a sanitary kitchen and protecting food against rodent or insect contamination. • Carriers must not be allowed to work as food handlers and should observe strict hygiene.

  19. THANK YOU

More Related