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IP-Flag WB Myc

IP-Flag WB Myc. WB Myc. WB Flag. + - + - - - - - - + + - - - +. XMyT1 Myc XMyT1mut Myc XCtBP Flag.

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IP-Flag WB Myc

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  1. IP-Flag WB Myc WB Myc WB Flag + - + - - - - - - + + - - - + XMyT1 Myc XMyT1mut Myc XCtBP Flag Figure 26 : Co-immunoprécipitation montrant que lemutant XMyT1XCtBP n’est plus capable de lier XCtBP. XMyT1- * XMyT1- XCtBP- Le mutant XMyT1XCtBP est constitué de trois mutations ponctuelles au niveau des trois motifs de recrutement de XCtBP. Ces trois motifs PGDLS, PENLS et TLDLS ont été mutés respectivement en PAAAS, PAAAS et TAAAS. Cette expérience montre que le mutant n’interagit plus avec XCtBP contrairement à la protéine XMyT1 sauvage. Légende : (*) bande de type artéfact.

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