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Chromosomes Objectives: Explain how karyotypes can be used to identify chromosomal abnormalities Identify the chromosomes responsible for a person’s sex Chromosomes Supercoiled complex of DNA and proteins DNA codes for LOTS of things The codes must be neatly organized
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Chromosomes Objectives: • Explain how karyotypes can be used to identify chromosomal abnormalities • Identify the chromosomes responsible for a person’s sex
Chromosomes • Supercoiled complex of DNA and proteins • DNA codes for LOTS of things • The codes must be neatly organized • The codes are organized into genes • Chromosomes are stacks of genes
Chromosomes • Chromosomes found in metaphase will have 2 chromatids and will be attached by a centromere • The chromatids are identical
Chromatin • DNA usually exists in chromatin • Chromatin is a uncoiled DNA and protein complex • Chromatin only supercoils when cells are entering mitosis or meiosis
Harvesting Chromosomes • Chromosomes develop from chromatin during prophase • They are clearly seen in metaphase since there is no nuclear membrane
Harvesting Chromosomes • Cells are frozen in metaphase • Chromosomes are then treated and stained • They are viewed under a microscope • A photograph is taken • The photograph is enlarged and the chromosomes are cut out
Karyotypes • Homologous chromosomes are paired • The pairs are arranged by size and in order with their centromeres aligned
Ploidy • Not all organisms have 23 pairs of chromosomes • 2N = diploid • N = haploid • 2N = 46 for humans • N = 23 for humans • Find the ploidy of the organisms listed
Sex Chromosomes • The first 22 pairs of chromosomes are called autosomes • The 23rd pair of chromosomes are called sex chromosomes • They code for whether the individual will be male or female • If the 23rd pair is homologous the individual is a female • If the 23rd pair is non-homologous the individual is a male
Sex Chromosomes • The chromosomes are referred to as X and Y • XX = female • XY = male • The Y has significantly less genes on it than the X
Sex Chromosomes Male Female
2n=46. Human We use karyotypes to find genetic disorders. And to determine the sex of the individual.
Karyotypes Homework • Find the pairs • Arrange them in pairs, in order of size, with the same line going through each pair’s centromeres • Paste the pairs in your comp book • Is the person a male or a female? • Is there a genetic disorder?
Karyotype Homework • Google karyotype • Print one of a non-human organism • Paste it to your compbook • Remember to identify what organism you chose
Socks Male FYI: Socks aren’t really organisms.