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Measurement with the Microscope

Measurement with the Microscope. Key Terms. Field of View (FOV) The diameter of circular image seen through a microscope. Usually measured in micrometers ( μ m). White Blood Cells seen through a microscope. FOV. …Key Terms. Fit Number

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Measurement with the Microscope

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  1. Measurement with the Microscope

  2. Key Terms • Field of View (FOV) • The diameter of circular image seen through a microscope. • Usually measured in micrometers (μm) White Blood Cells seen through a microscope FOV

  3. …Key Terms • Fit Number • The number of times a specimen will fit across the field of view. i.e. 2, 4, 3.5. • What is the fit number of the white blood cell shown below…?

  4. …Key Terms • …Fit Number • This cell will fit across the field of view approximately 5.3 times. • Fit number is 5.3

  5. …Key Terms • Scaled Magnification (SM) • Scaled magnification refers to how much bigger your drawing is than the actual image • If the actual image is 0.2 mm and your drawing is 20 mm, then SM = 100X • SM is NOT the same as ‘magnification’.

  6. Formulas How to calculate an unknown Field of view (FOV) Determine FOV on low power as instructed by your teacher Unknown FOV size = known FOV on low power X magnification on low power magnification at unknown FOV How to estimate the size of a specimen in a field of view size of specimen (in µm) = field size (in µm) “fit number” How to determine the scaled magnification scaled magnification = diagram size (mm) actual size (mm) Don’t forget to use the same units within the same formula!

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