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MRI GUIDED FOCUSED ULTRASOUND ACTIVATED NANO ENCAPSULATED TARGETED DRUG DELIVERY

MRI GUIDED FOCUSED ULTRASOUND ACTIVATED NANO ENCAPSULATED TARGETED DRUG DELIVERY. Doudou Xu PhD Student Institute for Medical Science and Technology (IMSaT) University of Dundee, Scotland. Outline. Introduction: Nanoporation Project Thermal Sensitive Sugar Based Carrier Nano-Sonication

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MRI GUIDED FOCUSED ULTRASOUND ACTIVATED NANO ENCAPSULATED TARGETED DRUG DELIVERY

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  1. MRI GUIDED FOCUSED ULTRASOUND ACTIVATED NANO ENCAPSULATED TARGETED DRUG DELIVERY Doudou Xu PhD Student Institute for Medical Science and Technology (IMSaT) University of Dundee, Scotland

  2. Outline • Introduction: Nanoporation Project • Thermal Sensitive Sugar Based Carrier • Nano-Sonication • Conclusion • Future Study

  3. Nanoporation Nanoporation is an interdisciplinary project • Pre-clinical oncology research • Cell biology • Magnetic Resonance guided Focused UltrasoundSurgery((MRgFUS) • Drug encapsulation nano-capsules • Targeted delivery of existing chemotherapy drugs • Nano-scale sonication research

  4. MRgFUS- Magnetic Resonance guided Focused UltrasoundSurgery • MRgFUS combines High Intensity Focused Ultrasound, that ablates a targeted tissue (non-invasively) monitored by MRI HIFU treated turkey breast

  5. The Nano-vehicles • Sugar based nanocarrier which can increase the solubility of hydrophobic drugs • The encapsulation is at molecular level • The carrier is triggered to release the drug at the tumor site Peptide Carrier Targeting group Encapsulated drug

  6. Thermo-Sensitive Carrier (I) • Encapsulation percentage around 95%; • 40-fold more uptake at 80°C than 37°C for Doxorubicin encapsulated carrier solution; DI=I[carrier]0-I[carrier]x

  7. Thermo-Sensitive Carrier (II) No Thermal Effect Thermal Effect Hyperthermia Release Drug Carrier • Hyperthermia increased penetration of Doxorubicin • Hyperthermia decreased binding constant and release Doxorubicin

  8. In vitro Sonication Machine (I)

  9. In vitro Sonication Machine (II)

  10. Nano-SonicationDoxorubicin Uptake Test Fluorescence Digraph 592.05nm With US (23.42) No US (11.79) Excitation λ =485nm Emission λ =592.05nm Slit=10

  11. Conclusion • Optimised carrier for Doxorubicin encapsulation efficiency is over 95% • CapsuTech sugar based carrier is sensitive to thermal effect • Hyperthermia mediated by sonication has been validated

  12. Future Studies • In vitro Viability test of Nano-Sonication by encapsulated Doxorubicin • In vivo studies in mice using ExAblate to release Doxorubicin from Nano-Carrier. • Encapsulation of additional drugs (Taxol, Camptothecin, and more) • Polymer level encapsulation research

  13. Our Team: Insightec, Ltd, Israel: Yoav Medan, D.Sc., Chief System Architect Alex Volovick, M.Sc, Scientist Osnat Dogadkin, M.Sc. Scientist Bjoern Gerold, PhD Student. Yelena Vachutinsky, PhD, scientist Capsutech, Ltd, Israel: Jallal Gnaim, PhD, CTO-Founder Fabian Arditti, PhD, R&D Manager, Dana Gurevich, PhD student. Doudou Xu, PhD student Nizar Mishael, CPO, Bus. Dev. Institute for Medical Science and Technology (IMSAT), and University of Dundee, UK: Prof. Andreas Melzer, MD, Director of IMSaT Prof. Sandy Cochran, PhD, Deputy Director Lijun Wang, PhD, Head of Biol.Lab Paul Prentice, PhD, Scientist Ritu Maliki, PhD, Post-Doc

  14. Acknowledgement • This research, Nanoporation, is an FP7 funded Marie Curie Industry-Academia Partnerships Pathway project, my studies are currently fully supported by Nanoporation • Supervisors in University of Dundee: Prof. Andreas Melzer, Dr. Lijun Wang • Supervisors in CapsuTech: Dr. Jallal Gnaim, Dr. Fabian Arditti

  15. Thanks for your attention!

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