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Evaluering af MRSA screening metoder i Skandinavien

Evaluering af MRSA screening metoder i Skandinavien. Sidsel Böcher, R. Skov Robert Smyth and Gunnar Kahlmeter Trond Jacobsen. Baggrund. Hvad er den optimale metode for detektion af MRSA i screenings prøver? Nye chromogene medier tilsat antibiotika synes “lovende”

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Evaluering af MRSA screening metoder i Skandinavien

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  1. Evaluering af MRSAscreening metoder i Skandinavien Sidsel Böcher, R. Skov Robert Smyth and Gunnar Kahlmeter Trond Jacobsen

  2. Baggrund • Hvad er den optimale metode for detektion af MRSA i screenings prøver? • Nye chromogene medier tilsat antibiotika synes “lovende” • Er de gode nok til at man ikke behøver at bruge opformering • Hvilken er den bedste til skandinaviske forhold? • Meget stor diversitet!

  3. Ref. Nahimana et al: Clinical Microbiology and Infection, Volume 12, Number 12, December 2006

  4. Sensitivity 14-28%

  5. Evaluation of 2 selective broths

  6. Formål: • Fælles skandinavisk afprøvning af forskellige chromogene plader +/- opformering. • Vi kan ikke afklare dette hver for sig • Vi er overladt til firmaers “løfter om guld og grønne skove” • Ved at gøre det fælles så skal hvert laboratorie levere få prøver

  7. Metode • På patienter hvor der er høj mistanke om at de er MRSA positive • Et ekstra sæt prøver for MRSA som sendes til et af de 3 centrale laboratorier • St. Olav, Växjö, SSI • i alt 30 patienter per laboratorie

  8. Procedure på central laboratorierne Swab is swirled in 1ml 0,9 % NaCl 100 mcl is used for: Direct inoculation SSI Semi-selective TSB broth 20mg/L Aztreonam 3,5mg/L Cefoxitin 2,5% Nacl Oxoid Biomerieux ? ? Control – staph agar, Biomerrieux MRSA ID MRSA Select MRSA SSI

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