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Cloning of potential promoter fragments Integration of the lacZ -fusion into the chromosome by

BcuI. IR. tnp. IR. pGP353. lacZ. ori colE1. 7652 bps. aacA-aphD. Bam. HI. Sma. I. Xma. I. Eco. RI. Promoter Analyses in Mycoplasma pneumoniae. Cloning of potential promoter fragments Integration of the lacZ -fusion into the chromosome by transposition

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Cloning of potential promoter fragments Integration of the lacZ -fusion into the chromosome by

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  1. BcuI IR tnp IR pGP353 lacZ ori colE1 7652 bps aacA-aphD Bam HI Sma I Xma I Eco RI Promoter Analyses in Mycoplasma pneumoniae Cloning of potential promoter fragments Integration of the lacZ-fusion into the chromosome by transposition Assay of LacZ activities on plates and in liquid media

  2. Analysis of the ldh Promoter HindIII HindIII ldh -10 -35 -35 -10 MPN673 -160 +81 -118 +81 -84 +81 -53 +81 -85 +1 -160 +81 The lacZ fusion reflects promoter activity

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