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Mussie Y. Habteselassie Crop and Soil Sciences UGA Griffin Campus

SOIL MICROBIOLOGY PROGRAM @ GRIFFIN CAMPUS. 3 rd YEAR REVIEW SPRING FACULTY MEETING, MACON, GA. Mussie Y. Habteselassie Crop and Soil Sciences UGA Griffin Campus. INTRODUCTION. Came to UGA in 2008 30% teaching / 70% research appointment Outline: Research program Sample projects

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Mussie Y. Habteselassie Crop and Soil Sciences UGA Griffin Campus

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  1. SOIL MICROBIOLOGY PROGRAM @ GRIFFIN CAMPUS 3rd YEAR REVIEW SPRING FACULTY MEETING, MACON, GA Mussie Y. Habteselassie Crop and Soil Sciences UGA Griffin Campus

  2. INTRODUCTION • Came to UGA in 2008 • 30% teaching / 70% research appointment • Outline: • Research program • Sample projects • Teaching Responsibility

  3. RESEARCH PROGRAM • Environmental Soil Microbiology Program 1. The ecology of human and animal pathogens in soils • 2. Environmental services of soil microbial community • 3. Microbial water quality

  4. Survival Transmission to produce 1. Ecology Of Human and Animal Pathogens In Soils Ecology of pathogens in soils • Use of model microorganisms • - Lux marked E. coli O157:H7/Salmonella spp • - GFP marked E. coli O157:H7/Salmonella spp • - Antibiotic resistant E. coli/Salmonella spp

  5. E. coli Persistence in the Rhizosphere of Fresh Produce Habteselassie, et al. 2010. Understanding the role of agricultural practices in potential colonization and contamination by E. coli in rhizospheres of fresh produce. JFP 73:2001-2009.

  6. 14 3 5 C Growth (d) C C T T T Control plants were not visible due to absence of E. coli O157:H7 lux. Tracking E. coli O157:H7 Activity and Transmission on Fresh Produce Root E. coli O157:H7 gfp

  7. Pathogen Persistence and Transmission during lettuce harvest • Co-PI on a grant from UC, Davis in collaboration with Dr. Cannon at the UGA Center for Food Safety: $338,414 • Two year project (Jan 2011-Dec 2012) • Soil organic matter accumulation on glove during harvest and its effect on pathogen persistence and transmission • Source tracking organic matter from soil vs lettuce

  8. N & C cycling Pollutant remediation 2. Environmental Services of Soil Microorganisms Soil microbes & their environmental services Approach - Molecular microbiology – targeting functional genes - Stable isotope techniques – process characterization

  9. A simplified Internal N cycle in soils Denitrification Mineralization Nitrification Organic N NH4+ NO3- Leaching Microbial Biomass Plants N Cycling Under Contrasting Agricultural Management Systems

  10. Ammonia Oxidizing Bacteria vs Ammonia Oxidizing Archaea

  11. Changes in Soil N Cycling Organisms Following Repeated Poultry Litter application Ammonia Oxidizing Bacteria Ammonia Oxidizing Archaea Control Plots Treatment Plots – Litter @ different rates Treatment Plots – Litter @ different rates Control Plots

  12. New Project On N Cycling • Functional and molecular diversity in nitrogen cycle enzymes under contrasting agricultural management systems. • USDA-AFRI Microbial Communities Program. Habteselassie, M.Y. (Co-PI):$499,894 • 5-year project; starts in July 2011 • Organic vs conventional farming systems

  13. Volatile Organic Compounds (VOC) Metabolizing organisms in Indoor Plants • Seed Money from the National Foliage Foundation (NFF) • VOCs from different sources such as carpets, computers • Some indoor plants efficient in removing VOCs • Microorganisms in the rhizosphere important players in VOCs removal • Survey in two homes in Athens, GA - >100 VOCs

  14. Isolation and Characterization PCR Cloning Sequencing Extraction, streaking & Isolation Identification & Characterization Priming

  15. TEACHING

  16. …TEACHING Natural Resources Science Major @ the Griffin Campus

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