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Optogenetic regulation of Ca 2+ signaling April 15, 2013. Class objectives: What is optogenetics? Mammalian rhodopsin. Biophysics of channelrhodopsin-2. Opto- XR Optically-induced dimerization. What is optogenetics?.
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Optogenetic regulation of Ca2+ signalingApril 15, 2013 Class objectives: What is optogenetics? Mammalian rhodopsin. Biophysics of channelrhodopsin-2. Opto-XR Optically-induced dimerization
What is optogenetics? “Optogenetics is the combination of optical and molecular strategies to monitor and control designated molecular and cellular activities in living tissues and cells using genetically encoded photosensitive proteins.”
Mammalian rhodopsin 11-cis-retinal
11-cis-retinylidene All-trans-retinylidene
Channelrhodopsin-2 is a light-activated cation channel Green alga Chlamydomonas reinhardtii Nagel G et al. PNAS 2003;100:13940-13945
Action spectra of channelrhodopsins Nagel G et al. PNAS 2003;100:13940-13945
ChR chimeras with different spectral properties Prigge M et al. J. Biol. Chem. 2012;287:31804-31812
Dual light excitation and ion selectivity for blue and green light-absorbing ChRs. Prigge M et al. J. Biol. Chem. 2012;287:31804-31812
Signalling specificity and in vivo functionality of Opto-XRs RD Airanet al. Nature (2009)
OptoXR: optogenetic control of intracellular signal transduction. RD Airanet al. Nature000, 1-5 (2009) doi:10.1038/nature07926
Light-induced translocation of PKCa FKF1-GI system
Rapid light-induced recruitment of a 5-ptase to the plasma membrane Idevall-Hagren O et al. PNAS 2012;109:E2316-E2323
Blue light-induced recruitment of a 5-phosphatase to the plasma membrane rapidly decreases KCNQ2/3 current. Idevall-Hagren O et al. PNAS 2012;109:E2316-E2323
Jia et al.(2011) Supplemental Movies Supplemental movies