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THE CONTROL OF FLOWERING. HOW DO PLANTS KEEP TRACK OF THE SEASONS?. PLANTS FLOWER AT DIFFERENT TIMES OF THE YEAR. WHICH ENVIRONMENTAL SIGNALS CONTROL FLOWERING?. HOW DO ENVIRONMENTAL SIGNALS BRING ABOUT THE TRANSITION TO FLOWERING?. FLOWERING IN ARABIDOPSIS. (B).
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THE CONTROL OF FLOWERING HOW DO PLANTS KEEP TRACK OF THE SEASONS? • PLANTS FLOWER AT DIFFERENT TIMES OF THE YEAR. WHICH ENVIRONMENTAL SIGNALS CONTROL FLOWERING? HOW DO ENVIRONMENTAL SIGNALS BRING ABOUT THE TRANSITION TO FLOWERING?
VEGETATIVE VS. FLOWERING SHOOT APEX IN ARABIDOPSIS VEGETATIVE APEX FLOWERING APEX
FLORAL EVOCATION • THE EVENTS OCCURRING IN THE SHOOT APEX THAT SPECIFICALLY COMMIT THE APICAL MERISTEM TO PRODUCE FLOWERS
QUADRUPLE MUTANT (ap1, ap2, ap3/pi, ag) RESULTS IN THE PRODUCTION OF LEAF-LIKE STRUCTURES IN PLACE OF FLORAL ORGANS
THE SHOOT APEX AND PHASE CHANGES THE SHOOT APICAL MERISTEM PASES THROUGH THREE DEVELOPMENTAL PHASES: 1. THE JUVENILE PHASE 2. THEADULT VEGETATIVE PHASE 3. THEADULT REPRODUCTIVE PHASE
JUVENILE AND ADULT FORMS OF ENGLISH IVY (Hedera helix) JUVENILE ADULT (GIBBERELLIN CAUSES REVERSION OF ADULT FORM TO JUVENILE FORM IN ENGLISH IVY)
PHOTOPERIODISM: MONITORING DAY LENGTH • PLANTS CAN USE CIRCADIAN RHYTHMS TO MEASURE DAYLENGTH
PLANTS CAN USE CIRCADIAN RHYTHMS TO MEASURE TIME Entrained rhythm Free running rhythm
DAY LENGTH VARIES WITH THE MONTH OF THE YEAR AND THE LATTITUDE, EXCEPT AT THE EQUATOR Day length = night length all year at the equator
THE DISCOVERY OF PHOTOPERIODISM • GARNER AND ALLARD (1920s) AT USDA LAB AT BELTSVILLE, MD STUDIED FLOWERING IN MARYLAND MAMMOTH TOBACCO PLANT. • MARYLAND MAMMOTH WAS A SINGLE GENE MUTANT TOBACCO THAT DIDN’T FLOWER IN THE SPRING OR SUMMER, LIKE WILD TYPE. • IT ONLY FLOWERED WHEN BROUGHT INTO THE GREEN HOUSE IN THE WINTER.
PLANTS CAN BE CLASSIFIED ACCORDING TO THEIR PHOTOPERIODIC RESPONSES • Short Day Plants (SDPs) flower when day length is shorter than a critical day length. • Long day plants (LDPs) flower when the day length is longer than a critical daylength. • Long-short-day plants (LSDPs) flower after a sequence of long days followed by short days. • Short-long-day plants (SLDPs) flower after a sequence of short days followed by long days. • Day-neutral plants (DNPs) are insensitive to daylength, Flowering is under internal developmental control.
THE SITE OF PERCEPTION OF THE PHOTOPERIODIC STIMULUS IS THE LEAF
EFFECT OF DAY LENGTH ON FLOWERING IN SDPs AND LDPs Critical day langth Critical day langth
NIGHT BREAK EXPERIMENTS DEMONSTRATE ROLE OF NIGHT PERIOD IN SDPs
NIGHT BREAK EXPERIMENTS ALSO DEMONSTRATE ROLE OF NIGHT PERIOD IN LDPs
THE LENGTH OF THE DARK PERIOD REGULATES THE PHOTOPERIODIC RESPONSES IN BOTH SDPs AND LDPs
NIGHT BREAKS GIVEN AT DIFFERENT TIMES DURING THE NIGHT PERIOD REVEAL CIRCADIAN RHYTHMICITY
ACCORDING TO THE COINCIDENCE MODEL, THE EFFECT OF THE NIGHT BREAK DEPENDS ON THE RHYTHM OF LIGHT SENSITIVITY (SDP Soybean)
PHYTOCHROME IS THE PIGMENT INVOLVED IN MEASURING THE NIGHT LENGTH
AS IN THE CASE OF SDPs, ACCORDING TO THE COINCIDENCE MODEL, THE EFFECT OF THE NIGHT BREAK DEPENDS ON THE RHYTHM OF LIGHT SENSITIVITY LDP Arabidopsis
VERNALIZATION: PROMOTING FLOWERING WITH COLD TREATMENT (40 DAYS AT 4C) Winter-annual Arabidopsiswith vernalization Winter-annual Arabidopsis without vernalization
VERNALIZATION: SITE OF PERCEPTION • THE SITE OF PERCEPTION OF • VERNALIZATION IS THE SHOOT TIP
DEVERNALIZATION • THE EFFECT OF COLD TEMPERATURE IN PROMOTING FLOWERING CAN BE REVERSED BY WARM TEMPERATURE (DEVERNALIZATION)
VERNALIZATION BLOCKS THE EXPRESSION OF THE GENE FLOWERING LOCUS C (FLC) Winter annual without cold Winter annual without cold, but with FLC mutation Winter annual after 40 cold days FLC mRNA
GRAFTING STUDIES GRAFTING STUDIES HAVE DEMONSTRATED THE ROLE OF A TRANSMISSIBLE STIMULUS PRODUCED BY LEAVES WHEN PHOTOPERIODICALLY INDUCED.
DEMONSTRATION OF LEAF-GENERATED FLORAL STIMULUS (FLORIGEN) IN THE SDP PERILLA Induced graft donor leaf Uninduced graft donor leaf
SUCCESSFUL TRANSMISSION OF FLORAL STIMULUS BY INTERGENERIC GRAFTING Scion: LDP Petunia hybrida, induced with long days. Stock: Nonvernalized, cold-requiring LDP Hyascyamus niger (henbane)