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A. B. PSA-luciferase. LEF-luciferase. Percent Control. Percent Control. 4000. 6000. DMSO. DMSO. DHT 10nM. DHT 10nM. 3000. 4000. 2000. 2000. 1000. 0. 0. GFP. Wnt1. AR. Wnt1+AR. GFP. Wnt1. AR. Wnt1+AR. C. D. PSA-Luciferase. LEF-luciferase. 120. 8000. DMSO. DMSO.
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A B PSA-luciferase LEF-luciferase Percent Control Percent Control 4000 6000 DMSO DMSO DHT 10nM DHT 10nM 3000 4000 2000 2000 1000 0 0 GFP Wnt1 AR Wnt1+AR GFP Wnt1 AR Wnt1+AR C D PSA-Luciferase LEF-luciferase 120 8000 DMSO DMSO 100 Nilutamide 10uM Nilutamide 10uM 6000 80 Percent Control Percent Control 4000 60 40 2000 20 0 0 GFP AR ART877A ARH874Y Wnt1+AR Wnt1+ART877A Wnt1+ARH874Y E F PSA-Luciferase LEF-luciferase 8000 120 DMSO DMSO Bicalutamide 10uM 100 Bicalutamide 10uM 6000 80 Percent Control Percent Control 4000 60 40 2000 20 0 0 ART877A +Wnt1 ARH874Y +Wnt1 ARW741L +Wnt1 ARW741C +Wnt1 GFP AR ART877A AR +Wnt1 ARW741C ARH874Y ARW741L