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PULSENET AMERICA LATINA 5 ta REUNION ANUAL 23 - 25 de octubre 2007 Buenos Aires, Argentina

PULSENET AMERICA LATINA 5 ta REUNION ANUAL 23 - 25 de octubre 2007 Buenos Aires, Argentina. PFGE-PulseNet experience – 2006/2007 Activity plan – 2007/2008 São Paulo State, Brazil. Ana Terezinha Tavechio Instituto Adolfo Lutz São Paulo, Brazil. EXPERIENCE 2006-2007.

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PULSENET AMERICA LATINA 5 ta REUNION ANUAL 23 - 25 de octubre 2007 Buenos Aires, Argentina

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  1. PULSENET AMERICA LATINA5ta REUNION ANUAL23 - 25 de octubre 2007 Buenos Aires, Argentina PFGE-PulseNet experience – 2006/2007 Activity plan – 2007/2008 São Paulo State, Brazil Ana Terezinha Tavechio Instituto Adolfo Lutz São Paulo, Brazil

  2. EXPERIENCE 2006-2007 • CHEF DR III acquisition • BioNumerics acquisition • Salmonella Gel analysis certification • Outbreaks PFGE analysis

  3. Salmonella/Shigella Outbreaks analyzed by PFGE (PulseNet) – 2006-2007 * August 2007

  4. Salmonella Outbreaks in São Paulo State - Brazil 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1135 668,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5 Lanes 1, 8, 15, Braenderup H9812; Lanes 2-7, Infantis (2007); Lanes 9-11, Bareilly (2005); Lanes 12-14, Corvallis (2004)

  5. Salmonella Outbreaks in São Paulo State - Brazil 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1135 668,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5 Lanes 1,8,15, Braenderup H9812; Lanes 2-4, Typhimurium; Lanes 5-7, Typhimurium; Lanes 9-10, Javiana; Lanes 11-12, Typhi * (2007); Lanes 13-14, Tennessee * (foodstuff) * Esporadic cases

  6. Salmonella Outbreaks in São Paulo State - Brazil 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1135 668,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5 Lanes 1, 8, 15, Braenderup H9812; Lanes 2-4 and 5-7, S. Enteritidis (2007); Lanes 9-11 and 12-14*, S. Enteritidis (2006) * cake

  7. Salmonella Outbreaks in São Paulo State - Brazil 1 2 3 4 5 6 7 8 9 10 KB 1135 668,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5 Lanes 1, 10, Braenderup H9812; Lanes 2-6, Typhi (outbreak) Lanes 7-9, Typhi (sporadic cases) (2006)

  8. Salmonella Outbreaks in São Paulo State - Brazil 1 2 3 4 5 6 7 8 9 10 1135 668,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5 Lanes 1, 6, 10, Braenderup H9812; Lanes 2-5 and 7,8, S. enterica 9,12:-:- (August 2007); Lane 9, S. enterica 1,9,12:-:- (esporadic case – September 2007)

  9. Shigella Outbreak in São Paulo State - Brazil 1 2 3 4 5 6 7 8 9 10 1135 668,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5 Lanes 1, 6, 10, Braenderup H9812; Lanes 2-5 and 7-9, Shigella sonnei (2007) * Lane 8, different PFGE and resistance profiles

  10. 2007 - 2008 Activity Plan • PFGE analysis • Outbreaks •  Extraintestinal Sporadic cases •  unusual serotypes • Salmonella Gel analysis

  11. 2007 - 2008 Activity Plan • PulseNetdatabase of Adolfo Lutz - SP • Salmonella • E. coli O157:H7 animal reservoirs • Participation on the PulseNet Projects

  12. THANK YOU! Ana Terezinha Tavechio Instituto Adolfo Lutz Av. Dr. Arnaldo, 351 – 9º andar Cerqueira Cesar – São Paulo – SP CEP 01246.902 – Brasil atavechio@ial.sp.gov.br atavecch@hotmail.com

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