luciferase cells

1. Application of Luciferase Cells What is Luciferase Cells? Luciferase Cell(English name: Luciferase) is the general name of the enzymes that can produce bioluminescence in nature. The most representative one is Luciferase in firefly, which is called Photinus pyrali.Fluorescence is produced by the oxidation of luciferin in the corresponding chemical reactions, and in some cases the reaction system also includes adenosine triphosphate (ATP).Without luciferase, luciferin reacts very slowly with oxygen, and the presence of calcium ions often accelerates the reaction further (similar to muscle contraction). Luciferin or luciferase is not a specific molecule, but rather a general term for all the substrates and corresponding enzymes that produce fluorescence, although they vary. Different luminescent organisms use different luciferase enzymes to catalyze different luminescent reactions.The best known bioluminescent organism is the firefly, which USES different luciferase enzymes from other bioluminescent organisms such as the firefly mushroom (Omphalotus oleariu') or many Marine organisms.In fireflies, the oxygen needed for the bioluminescent response is pumped into a tube called the abdominal trachea. Some organisms, such as the elater, contain a variety of different

2. luciferase enzymes that catalyze the production of different colours of fluorescence from the same substrate.There are more than 2,000 species of firefly and many more in the elateroidea (including fireflies, elaterites and related insects), so their luciferase is useful for molecular phylo Application of Luciferase Cells Luciferase can be produced in the laboratory using genetically engineered methods and is used in a variety of experiments. Luciferase genes can be synthesized and inserted into living organisms or transfected into cells. Researchers have used genetic engineering to make luciferase in mice, silkworms, potatoes and other organisms. Indirectly in vitro imaging is a powerful means of research, to analyze the whole animal body cells in the community: different types of cells (bone marrow stem cells and T cells, etc.) tag (expression) luciferin enzyme, can use the high sensitivity of the CCD camera to live animals to observe and do not harm to the animals themselves. Fluorescence can be emitted by adding the correct luciferin substrate to luciferase, and the emitted photon can be detected by light sensitive elements such as fluorescence detector or improved optical microscope. This makes it possible to observe the course of many life processes, including infection.For example, luciferase can be used to detect whether red blood cells in blood Banks are beginning to break down. Forensic scientists can use a solution containing luciferase to detect traces of blood left at a crime scene.Hospitals use luciferase light to detect specific diseases. Luciferase can also be used as a "reporter protein" in molecular biology studies, for example, to detect transcription of specific promoters in cells transfected with luciferase or to detect intracellular ATP levels. The technique is called reporter Assay or Luciferase Assay. Luciferase is a heat sensitive protein and is often used to study the protective capacity of heat shock proteins during protein thermal degeneration.