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Gemini for Miniprep - High Commands

Copy Plate Replicates Merge Plates Serial dilution. Gemini for Miniprep - High Commands. High Commands. A High Level Command is like a “mini” method. Gemini carries out a no. of pipetting steps & also performs washes and DiTi changes within one command.

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Gemini for Miniprep - High Commands

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  1. Copy Plate • Replicates • Merge Plates • Serial dilution Gemini for Miniprep - High Commands Gemini Training Page 1

  2. High Commands AHigh Level Command is like a “mini” method. Gemini carries out a no. of pipetting steps & also performs washes and DiTi changes within one command. Multi Dispense and Single Dispense modes. Gemini does not allow a mixed tip configuration when using High Level Commands Gemini Training Page 2

  3. High Commands - Copy Plate Copy Plate allows you to make Daughter plates from a mother plate Copy plate must have the same number of source wells as copy wells Gemini Training Page 3

  4. High Commands - Copy Plate Gemini Training Page 4

  5. High Commands - Replicates The Replicate Command allows you to make multiple copies of a Source well in a Destination rack. This Command is most often used for adding Reagent to a Plate. Gemini Training Page 5

  6. High Commands - Replicates Gemini Training Page 6

  7. High Commands - Replicates using System Liquid Gemini Training Page 7

  8. High Commands - Replicates Gemini Training Page 8

  9. High Commands - Replicates Gemini Training Page 9

  10. High Commands - Replicates AB to AABB Multipipetting Gemini Training Page 10

  11. High Commands - Replicates AB to ABAB Multipipetting Gemini Training Page 11

  12. High Commands - Replicates AB to BBAA Multipipetting Gemini Training Page 12

  13. High Commands - Replicates AB to BABA Multipipetting Gemini Training Page 13

  14. Exercise FIVE Gemini Training Page 14

  15. High Commands - Merge Plates Merge Plates allows you to create a destination rack by placing wells from one or more Source racks into one destination rack. This command is most often used for merging 4 x 96 well MTP’s into 1 x 384 well MTP Gemini Training Page 15

  16. High Commands - Merge Plates Gemini Training Page 16

  17. High Commands - Merge Plates Use the “Distance of destination wells” to Reformat the layout of the Source wells in the Destination plate Gemini Training Page 17

  18. WELL 1 A1 A1 Source 1 Source 3 H12 H12 X1,Y1 A1 A1 Source 2 Source 4 H12 H12 WELL 384 High Commands - Merge Plates(4 x 96 well plates to 1 x 384 well plate Gemini Training Page 18

  19. High Commands - Serial Dilution Gemini Training Page 19

  20. High Commands - Serial Dilution • A Command to perform a serial dilution with two or more steps. • Every dilution step has the same dilution ratio. Gemini Training Page 20

  21. High Commands - Serial Dilution When using “Diluent for Each Step”, diluent for a single step will be aspirated at a time. When using “Diluent for All Steps”, the diluent will be aspirated only once, in sufficient volume for all following dilution steps. Gemini Training Page 21

  22. High Commands - Serial Dilution The Destination volume is the volume of Diluent added & so also the final volume left in the well Gemini Training Page 22

  23. High Commands - Serial Dilution When using The Mode “Diluent for all steps” you must define the no. of copies per Aspiration. Gemini Training Page 23

  24. Exercise SIX • Merge Plates Merge 4 x 96 well plates into 1 x 384 well plate using the High level command • with and without high density command. Gemini Training Page 24

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