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Tip60 mRNA. 1.2. 1.0. 0.8. 0.6. Relative expression levels. 0.4. 0.2. 0. RKO. LoVo. HIEC. HT29. HCT8. Caco-2. SW620. HCT116. p400 mRNA. 4.0. 3.0. 2.0. Relative expression levels. 1.0. 0. RKO. LoVo. HIEC. HT29. HCT8. Caco-2. SW620. HCT116.
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Tip60 mRNA 1.2 1.0 0.8 0.6 Relative expression levels 0.4 0.2 0 RKO LoVo HIEC HT29 HCT8 Caco-2 SW620 HCT116 p400 mRNA 4.0 3.0 2.0 Relative expression levels 1.0 0 RKO LoVo HIEC HT29 HCT8 Caco-2 SW620 HCT116 Supplemental Data 3: Total RNA from the indicated cell lines was extracted, reversed transcribed and analyzed by real time PCR for the expression of Tip60, p400 and control GAPDH mRNA. The amount of Tip60 or p400 mRNA was then divided by the amount of control GAPDH mRNA and calculated relative to 1 for normal HIEC cells. The mean and standard deviation from 3 independent experiments are shown. Supplemental Data 3