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Supplemental Data

Supplemental Data. Rathinam et al: Changes in the serum proteome associated with lung cancer and their reversal following tumor resection. Table S1: Patient Information.

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Supplemental Data

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  1. Supplemental Data Rathinam et al: Changes in the serum proteome associated with lung cancer and their reversal following tumor resection Table S1: Patient Information. Fig A: Apolipoprotein C1 ImmunoSELDI. The experiment was performed as descibed in reference 7. The antibody used was a mouse monoclonal to human apolipoprotein C1 purchased from Chemicon International Inc (MAB1064). Fig B: Lsozyme C ImmunoSELDI. The antibody used was a rabbit polyclonal to human lysozyme C purchased from Abcam (ab2408) Figs C & D: MS/MS spectra used to identify Lysozyme Fig E-I: MS/MS spectra used to identify Transthyretin Fig J: Transthyretin ImmunoSELDI. The antibody used was a rabbit polyclonal to human transthyretin purchased from from Abcam (ab16006). Figs K & L: MS/MS spectra used to identify th4 m/z 5080 fragment of transthyretin. Fig M: Accurate mass determination of the m/z 5080 fragment of transthyretin. Fig N: M/Z 5080 ImmunoSELDI. The same antibody was used as in the intact transthyretin ImmunoSELDI (Fig J). Fig O: MS/MS spectra used to identify the m/z 3273 and 3289 fragments of ITIH4. The top spectrum/peptide is common to both fragments and the annotated spectra below show both the oxidised and non-oxidised verions of the methioinine containing peptide.

  2. Fig A 13000 14000 15000 16000 Lysozyme C ImmunoSELDI Fig B A Whole serum * B Depleted serum Intensity C Eluted proteins * 13000 14000 15000 16000 m/z

  3. Lysozyme MS/MS Fig C R.STDYGIFQINSR.Y R.GISLALWMC*LAK.W * = carboxyamidomethylation Fig D

  4. Transthyretin MS/MS Fig E K.TSESGELHGLTTEEEFVEGIYK.V Fig F R.GSPAINVAVHVFR.K

  5. Fig G K.AADDTWEPFASGK.T Fig H R.KAADDTWEPFASGK.T

  6. Fig I K.ALGISPFHEHAEVVFTANDSGPR.R Transthyretin ImmunoSELDI Fig J Whole serum A Depleted serum Intensity B Eluted proteins C 13000 14000 15000 m/z

  7. ‘5080’ MS/MS Fig K K.ALGISPFHEHAEVVFTANDSGPR.R Fig L A.ALLSPYSYSTTAVVTNPK.E

  8. m/z 5080(Transthyretin Fragment) ImmunoSELDI Whole serum M1 Intensity Depleted serum Eluted proteins * M2 m/z Fig M Fig N

  9. ‘3273/3289’ MS/MS MNFRPGVLSSR y1-4 b1-2 y1-3 b1-7 y1-7 b1-4 y1-2 y1-6 M*NFRPGVLSSR y1-7 y1-3 y1-4 b1-4 b1-2 b1-7 y1-6 R.PGVLSSRQLGLPGPPDVPDHAAYHPF.R (common to both) Fig O

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