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Supplemental data 1 Supplemental data 1. Effect of SBHA on cell cycle distributions in HeLa cells. Exponentially growing cells were treated with the designed doses of SBHA for 24 hours. The graph indicates the changes of cell cycle phase distributions, assessed by DNA flow cytometric analysis.
Supplemental data 2 Supplemental data 2. Effect of SBHA on intracellular DCF (ROS) level in HeLa cells. Exponentially-growing cells were treated with the designed dose of SBHA for the indicated times. DCF (ROS) levels in HeLa cells were measured using a fluorescence meter (SynergyTM 2, BioTekInstruments Inc.,Winooski, VT). (RFU=Relative Fluorescence emission Unit)
Supplemental data 3 Supplemental data 3. Effect of NAC, Vit. C and BSO on intracellular DCF (ROS) level in SBHA-treated HeLa cells. Exponentially-growing cells were treated with the designed dose of SBHA with or without NAC, Vit. C and BSO for the indicated times. ROS levels in HeLa cells were measured using a fluorescence meter (SynergyTM 2, BioTek Instruments Inc.). (RFU=Relative Fluorescence emission Unit)
Supplemental data 4 - + + - - + - - + + - - - - - - + + Supplemental data 4. Effect of BSO on GSH level in SBHA-treated HeLa cells. Exponentially-growing cells were treated with the 30 μM SBHA with or without 10 μM or 200 μM BSO for 72 hours. GSH level in HeLa cells were measured using a fluorescence meter (SynergyTM 2, BioTek Instruments Inc.). SBHA 30 μM : BSO 10 μM : BSO200 μM :
