SURVEY OF BIOCHEMISTRY Course Review

1. SURVEY OF BIOCHEMISTRYCourse Review

2. PRS-1 • Which amino acid contains a ring system? • Phe • Ile • Val • Gly

3. PRS-1 • Which amino acid contains a ring system? • Phe • Ile • Val • Gly

4. PRS-2 • Which enzyme is an oxidoreductase? • Hemoglobin • Enolase • GAP Dehydrogenase • Aconitase

5. PRS-2 • Which enzyme is an oxidoreductase? • Hemoglobin • Enolase • GAP Dehydrogenase • Aconitase What gets oxidized? What gets reduced?

6. PRS-3 • In mixed inhibition ___________. • Vmax increases and KM increases • Vmax decreases and KM increases • Vmax stays the same and KM increases • Vmax decreases and KM stays the same

7. PRS-3 • In mixed inhibition ___________. • Vmax increases and KM increases • Vmax decreases and KM increases • Vmax stays the same and KM increases • Vmax decreases and KM stays the same

8. PRS-4 • A fatty acid with 18 carbons and no double bonds in the alkyl chain is called: • Stearic acid • Palmitic Acid • Linoleic acid • Arachidonic acid

9. PRS-4 • A fatty acid with 18 carbons and no double bonds in the alkyl chain is called: • Stearic acid • Palmitic Acid • Linoleic acid • Arachidonic acid

10. PRS-5 • An alpha helix is stabilized by: • Hydrogen bonds between the side chains of the amino acids • Hydrogen bonds between the carbonyl oxygens and the N-H group of the adjacent residue • H-bonds between the carbonyl oxygens and the N-H group of the (n+3)th residue • H-bonds between the carbonyl oxygens and the N-H group of the (n+4)th residue

11. PRS-5 • An alpha helix is stabilized by: • Hydrogen bonds between the side chains of the amino acids • Hydrogen bonds between the carbonyl oxygens and the N-H group of the adjacent residue • H-bonds between the carbonyl oxygens and the N-H group of the (n+3)th residue • H-bonds between the carbonyl oxygens and the N-H group of the (n+4)th residue

12. PRS-6 • In ion exchange chromatography, the protein captured by the resin is eluted with: • Salt • Water • Imidazole • Diethylaminoethyl groups

13. PRS-6 • In ion exchange chromatography, the protein captured by the resin is eluted with: • Salt • Water • Imidazole • Diethylaminoethyl groups

14. PRS-7 • What reaction follows GAP dehydrogenase in glycolysis? • Phosphoglucomutase • Phosphofructokinase • Phosphoglycerate kinase • Phosphoglycerate mutase

15. PRS-7 • What reaction follows GAP dehydrogenase in glycolysis? • Phosphoglucomutase • Phosphofructokinase • Phosphoglycerate kinase • Phosphoglycerate mutase

16. PRS-8 • What is Dansyl Chloride used for? • End group analysis in peptide sequencing • Staining polyacrylamide gels • Determining protein concentration • Reducing disulfide bonds

17. PRS-8 • What is Dansyl Chloride used for? • End group analysis in peptide sequencing • Staining polyacrylamide gels • Determining protein concentration • Reducing disulfide bonds

18. PRS-9 • Which amino acids make up the catalytic triad of serine proteases? • His, Lys, Arg • Ser, His, Asp • Asp, Cys, Ser • Lys, Ser, Asp

19. PRS-9 • Which amino acids make up the catalytic triad of serine proteases? • His, Lys, Arg • Ser, His, Asp • Asp, Cys, Ser • Lys, Ser, Asp

20. PRS-10 • Which of the following is NOT a disaccharide? • Lactose • Maltose • Sucrose • Mannose

21. PRS-10 • Which of the following is NOT a disaccharide? • Lactose • Maltose • Sucrose • Mannose

22. PRS-11 • How many fused rings are found in cholesterol? • One • Two • Three • Four

23. PRS-11 • How many fused rings are found in cholesterol? • One • Two • Three • Four

24. PRS-12 • Which of the following cannot serve as a nucleophile? • Ser • His • Asp • Zn2+

25. PRS-12 • Which of the following cannot serve as a nucleophile? • Ser • His • Asp • Zn2+