Ei-2xAL22

1. dna dna dna insect dark light insect dark insect light dark light control control control A- Kas-1xAL41 Ei-2xAL52 Ei-2xAL22 1.0 1.0 1.0 0.9 0.9 0.9 0.8 0.8 0.8 0.7 0.7 0.7 0.6 0.6 0.6 0.5 0.5 0.5 0.4 0.4 0.4 0.3 0.3 0.3 dna dna dna control insect insect insect light light light flowers flowers control flowers control Kas-1xAH12 B- Ka-0xAH4 Kas-1xAH4 1.0 1.0 1.0 1.0 1.0 1.0 0.9 0.9 0.9 0.9 0.9 0.9 0.8 0.8 0.8 0.8 0.8 0.8 0.7 0.7 0.7 0.7 0.7 0.7 0.6 0.6 0.6 0.6 0.6 0.6 0.5 0.5 0.5 0.5 0.5 0.5 0.4 0.4 0.4 0.4 0.4 0.4 0.3 0.3 0.3 0.3 0.3 0.3 0.2 0.2 0.2 0.2 0.2 0.2 0.1 0.1 0.1 0.1 0.1 0.1 0.0 0.0 0.0 0.0 0.0 0.0 Supplementary figure 1: Box plots showing relative expression level in interspecific hybrid. Data are shown separately for each parental genotype combination. For each cross, the Yaxis of the box plots indicates the relative expression level. The red line indicates the expected value for equal promoter activity of both parental cis-regulatory regions. A- A. thaliana – A. lyrata hybrids; B- A. thaliana – A. halleri hybrids. Sample size for each genotype and each treatment are reported in suppl. table 3. AL22, AL52 and AH4 are heterozygotes and their cis-regulatory alleles are functionally different (see Fig. 4).