330 likes | 544 Views
mitochondria. Mitochondria in Kidney: Black staining of the mitochondria More black staining means more mitochondria Bottom of kidney slide lots = more transport Top has little = lighter = less transport Mitochondria have cristae
E N D
Mitochondria in Kidney: • Black staining of the mitochondria • More black staining means more mitochondria • Bottom of kidney slide lots = more transport • Top has little = lighter = less transport • Mitochondria have cristae • Mitochondria are rod like and about 0.25 um in diameter (can be 7 um in length)
Ribosomes and RER in spinal nervous tissue • Cytoplasm is filled with this blue staining material = RER and Ribosomes • The stain actually is staining the ribosomal RNA in the ribosomes on the RER • The nucleolus is also highly stained because this is where ribosomes are constructed • ***Do not rely on color to make determinations because the parts being stained could be dispersed, concentrated, etc which will cause different coloring • Should probably understand what each stain marks/ binds to
Golgi apparatus in Dorsal Root Ganglion Slide • The Nucleus is a clear color • Surrounded by black dots which may look crescent shaped • These are the Golgi apparatuses • Golgi are perinuclear (only around the nucleus) • Can see very discretely unlike the ribosomes • Specific silver stain that only differentiates Golgi
Microfilaments and Skeletal Muscle • Some microfilaments stain dark and then adjacent to this there are other microfilaments that stain lighter which causes the appearance of bands • Direction of Contraction • Striated tissue seen as light, vertical bands. Microfilaments actually run horizontally • A-Band, Seen as darker vertical band, composed of myosin. (Stationary during contraction) • All considered inclusions. Not membrane bound and low metabolic activity
Nuclei • Use nuclei to identifiy cell type • Use size, location, and shape to determine cell type
Nucleus and the Liver • Can see difference in the nucleus of the hepatocyte and the endothelial cell
Neurovascular bundle and nucleus (Nerve, artery, and vein run together)
Skeletal muscles and nuclei • Nuclui located on the periphery • Can have many nuclei • Also see microfilaments (pink) in muscle cell
Blastula and Mitosis (Nucleus examination) • Black dots are yolk in blastula ignore it
Unit 1 Exam Microanatomy Lab Practical Practice Questions Cytology, Epithelium, and Connective Tissue
What Cellular Organelle can be visualized by this Regaud’s Stain for Iron (blue and Black arrows?
What is the function of the organelle stained within the red circle?
What membrane bound organelle indicated by the blue arrows appears as these dark moon shaped structures in this type of staining and is involved in packaging of proteins into vesicles?
Which phases of the cell cycle are indicated by the colored numbers 1 2 3 4 5
What is the organelle that is show in this Micrograph that is involved in the production of protein?
The coated pits and coated vesicles in this micrograph are surrounded by what protein?
These cytoplasmic granules indicated by the green arrows are utilized by the mitochondria because they supply energy in the form of what?
This double membraned organelle regulates signals to the entire cell what structures indicated by the red and green arrows allow them send out these signals?