1 / 16

Avena genetic resources for quality in human consumption (AVEQ)

Avena genetic resources for quality in human consumption (AVEQ). Partner 1 2 IHAR -PIB Laboratory of Quality Evaluation of Plant Materials Institute of Plant Breeding and Acclimatization - National Research Institute , Radzików, PL 05 - 870, Poland.

lang
Download Presentation

Avena genetic resources for quality in human consumption (AVEQ)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Avena genetic resources for quality in human consumption (AVEQ) Partner 12 IHAR -PIB Laboratory of Quality Evaluation of Plant Materials Institute of Plant Breeding and Acclimatization - National Research Institute, Radzików, PL 05-870, Poland AVEQ Final Meeting – BucharestOctober 19-21, 2010

  2. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre The objective: Characterise carbohydrates - starch and dietary fibre in genetic resources of Avena. AVEQ Final Meeting – Bucharest October 19-21, 2010

  3. WP 7. Task 1, Sub-task 4 . Analysis of starch - selected method -amylase/amyloglucosidase method: ICC Standard no. 128/1and168 or AACC 76-13 or AOAC 996.11 AVEQ Final Meeting – Bucharest October 19-21, 2010

  4. WP 7. Task 1, Sub-task 4 . Analysis of dietary fibre - selected method Enzymatic-Chemical Uppsala Method (AOAC 994.13 or AACC 32-25) Gas chromatographic-colorimetric-gravimetric procedure • TDF = neutral sugar residues + uronic acids + Klason lignin • Principle • 1. Measurement of NSP as neutral sugar residues by GLC • (Englyst and Cummings procedure, 1988) • starch removal by specific enzymes • precipitation of soluble polymers by ethanol • hydrolysis of polysaccharides to monosaccharides with H2SO4 • reduction and derivatisation • 2. Measurement of uronic acid by colorimetry (Scottmethod, 1979) • 3. Measurement of Klason lignin by gravimetric method as the sulphuric acid resistant residue (Theander et al.,1995)

  5. Dietary Fibre Constituents I. Non-starch polysaccharides & oligosaccharides Cellulose Non-cellulosic polysaccharides • Polyfructoses • inulin • oligofructans • Galactooligosugars • Hemicelluloses • arabinoxylans • arabinogalactans • β-glucans • Pectins • Gums • Mucilages IV. Substances associated with NSP & lignin complex in plants II. Analogous Carbohydrates Waxes Phytates Cutin Suberin Saponins Tannins Resistant starch Synthesized carbohydr. Indigestible dextrins III. Lignins AVEQ Final Meeting – Bucharest October 19-21, 2010

  6. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre Material analysed from the year 2008 - 60 samples of selected accessions from trial in Bulgaria - 61 samples of selected accessions from trial in Estonia - 2 samples of wild accessions from trial in Estonia - 6 samples of wild accessions from trial in Poland - 53 samples of standards from trial in Estonia Total no of accessions: 182 AVEQ Final Meeting – Bucharest October 19-21, 2010

  7. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre I set - selected 60 accessions from Bulgaria trial Range 51.9 – 64.1%; avg – 56.3%; CV – 4.3%

  8. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre I set - selected 60 accessions from Bulgaria trial TDF - range 9.4–18.2% avg - 12.0%; CV - 10% 5.0 2.6 6.1 14.1 NSP - range 6.1–14.1% avg - 8.8 %; CV - 13% Uronic acids - range 0.14–0.32% avg – 0.23%; CV - 18% Lignin - range 2.6–5.0% avg - 3.3%; CV - 13%

  9. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre II set - selected 61 accessions from Estonia trial Range 48.9 – 64.9%; avg – 58.7%; CV – 5.9%

  10. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre II set - selected 61 accessions from Estonia trial TDF - range 9.0–15.8% avg - 11.2%; CV - 12% 4.4 1.8 11.8 6.5 NSP - range 6.5–11.8% avg - 8.2 %; CV - 13% Uronic acids - range 0.12–0.24% avg – 0.17%; CV - 16% Lignin - range 1.8–4.4% avg - 2.8%; CV - 22%

  11. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre II set - selected 61 accessions from Estonia trial plus 2 wild Avena samples TDF - range 9.0–15.8% avg - 11.2%; CV - 12% NSP - range 6.5–11.8% avg - 8.2 %; CV - 13% Uronic acids - range 0.12–0.24% avg – 0.17%; CV - 16% Lignin - range 1.8–4.4% avg - 2.8%; CV - 22%

  12. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre Polish wild species AVEQ Final Meeting – Bucharest October 19-21, 2010

  13. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre - standards

  14. WP 7. Task 1, Sub-task 4 . Relationship between NSP on TDF content TDF [% DM] NSP [% DM] AVEQ Final Meeting – Bucharest October 19-21, 2010

  15. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre • Plans for the next four months: • Additional standard samples (53 samples of 11 cultivars from field experiment in Estonia) at present are analyzing for dietaryfibreand they shouldbe completed within 10 day from now. • Analyses for starch and TDF (NSP only) in 70 selected accessions from trial inPoland (2008) and France, Sweden and Italy (2009). AVEQ Final Meeting – Bucharest October 19-21, 2010

  16. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre • This work has been carried out by : • Anna Fraś • Olga Jablonka • Danuta Boros • With appreciable help of two technicians: • Magdalena Bodzon • Monika Skonieczna AVEQ Final Meeting – Bucharest October 19-21, 2010

More Related