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The Pathological Protein Ch 4, 5

The Pathological Protein Ch 4, 5. BY Nicholas Toney. Connecting The Holes. An Uncanny Resemblance Hadlow - was Scrapie transmissible? Studying Scrapie Cuille and Chelle 15 month incubation period. Trying Transmissions. Hadlow's Letter and Patuxent Georgette's Sacrifice

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The Pathological Protein Ch 4, 5

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  1. The Pathological Protein Ch 4, 5 BY Nicholas Toney

  2. Connecting The Holes • An Uncanny Resemblance • Hadlow- was Scrapietransmissible? • Studying Scrapie • Cuilleand Chelle • 15 month incubation period

  3. Trying Transmissions • Hadlow's Letter and Patuxent • Georgette's Sacrifice • "Their loss... was felt by the staff“ • The Kuru-CJD Link • CJD experiments at Patuxent • Establishment of TSEs

  4. End of an Epidemic • Cannibalism in Kuru • Cases of Kuru in early 2003 • Implications? • Nobel Worthy • Gajdusek "fretted" that Zigas and Gibbs were not included

  5. The Birth of the Prion • The Central Dogma

  6. A Tough Invader • TikvahAlper • Radiation experiments • High energy electrons • Ultraviolet radiation • The Elusive Agent • Central Dogma maintained • Thoertical model proposed by mathmeticain

  7. TSE's New Player • Pruisner Persevered • New Methodology • Isolation of the Agent • Protease Sensitivity • Filtered at 50-100kDa250 Amino Acid Minimum • Maximum of 50 nucleotides

  8. The Virus Paradox

  9. The Prion Proposal • Controversy over Pruisner'swork • Frank Masiarze refused Co-Authorship • Nature and Lancet soon published rebuttals • Fatal Filaments Patricia Merz • Filaments associated with Scrapie • Manuelidis • CJD confirmed Amyloid plaques?

  10. The Normal and the Diabolical • DNA sequencing as a tool to find the causative agent • Pruisiner found that the scrapieagent existed in healthy and infected brains • Proteinase K - protein differentiation

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