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Tom Jenkins, Professor Department of Animal & Veterinary Sciences

Rumen Transformations of Lipid. Tom Jenkins, Professor Department of Animal & Veterinary Sciences. Rumen Transformations of Lipid Review of Dietary Lipid. Fatty acids are most important lipid fraction . Majority of fatty acids are bound to glycerol as simple or compound lipids .

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Tom Jenkins, Professor Department of Animal & Veterinary Sciences

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  1. Rumen Transformations of Lipid Tom Jenkins, Professor Department of Animal & Veterinary Sciences

  2. Rumen Transformations of Lipid Review of Dietary Lipid • Fatty acids are most important lipid fraction. • Majority of fatty acids are bound to glycerol as simple or compound lipids. • Majority of fatty acids are unsaturated.

  3. Rumen Transformations of Lipid Learning Objectives • What happens to dietary lipid as it passes from the diet to the intestines and is metabolized by rumen microorganisms? • What are the intermediates and end products that accumulate in the rumen from lipid metabolism? • How does lipid transformations in the rumen affect animal performance?

  4. Rumen Transformations of Lipid Compounds Extracted in Fat Test? Simple Lipolysis Compound PUFA Biohydrogenation MUFA Microbial Lipid Synthesis VFA

  5. Rumen Transformations of Lipid How Does Dietary Lipid Change as it Passes Through the Rumen? 47.9 lb. DMI 60% Forage (mainly corn silage, 5 lbs. alfalfa hay) From CPM for Dairy v.3.0.8

  6. Rumen Transformations of Lipid Rumen FA Transformations Small Intestine Esophagus Rumen Large Intestine Rumen Omasum Reticulum Abomasum From CPM for Dairy v.3.0.8

  7. Rumen Transformations of Lipid Biohydrogenation H H H H H H H H H H H H H H H H H O H C C C C C C C C C C C C C C C C C C OH H H H H H H H H H H H H H Unsaturated (18:2) Stearic (18:0)

  8. Rumen Transformations of Lipid Biohydrogenation Unsaturated Fat H H H H H C C C C C H H H

  9. Rumen Transformations of Lipid Biohydrogenation H H H H H C C C C C H H H H H

  10. Rumen Transformations of Lipid Biohydrogenation Saturated Fat H H H H H C C C C C H H H H H

  11. Rumen Transformations of Lipid Biohydrogenation Unsaturated (18:2) CLA (18:2) trans (18:1) Stearic (18:0)

  12. Rumen Transformations of Lipid Biohydrogenation Unsaturated Fat H H H H H C C C C C H

  13. Rumen Transformations of Lipid Biohydrogenation H H H H H C C C C C H H

  14. Rumen Transformations of Lipid Biohydrogenation CLA H H H H C C C C C H H

  15. Rumen Transformations of Lipid CLA Intermediates in Digesta Linoleic (18:2) cis–9 trans–11 (18:2) trans–11 (18:1) Stearic (18:0) From Teter, B.B., and T.C. Jenkins. 2006. In Advances in Conjugated Linoleic Acid Research, Vol. 3

  16. Rumen Transformations of Lipid Point 1–Diet Can Change BH Linoleic Acid (cis–9, cis–12 C18:2) Altered Pathways Conjugated Linoleic Acid (cis–9, trans–11 CLA) Conjugated Linoleic Acid Isomers trans–11 C18:1 trans–C18:1 Isomers Stearic (C18:0) Stearic (C18:0)

  17. Rumen Transformations of Lipid Point 2 MINOR intermediates of biohydrogenation can have MAJOR effects on animal performance and human health

  18. Rumen Transformations of Lipid Impact of Rumen Lipid Metabolism • Affects human health • Meat and milk higher in saturated fat • Bioactive metabolites • Anti-carcinogenic • Anti-atherogenic • Enhance immune system

  19. Rumen Transformations of Lipid Impact of Rumen Lipid Metabolism • Affects animal performance • Supply of essential fatty acids– reproduction • Fatty acid antimicrobial effects–production • Bioactive metabolites • Milk fat depression

  20. Rumen Transformations of Lipid Question Match each of the below from the list of answers shown on the right. One answer per blank. ___ Abbreviation of the major fatty acid consumed by dairy cows ___ Increases FFA in rumen contents ___ Abbreviation of the CLA normally produced in the rumen ___ Greatly reduces the supply of essential fatty acids to body tissues Biohydrogenation cis–9, trans–11 18:2 Lipolysis cis–9, cis–12 18:2 trans–11 C18:1 Peroxidation

  21. Rumen Transformations of Lipid Question Which of the below is the best substrate for the synthesis of CLA in the rumen? Omega–9 fatty acids Essential fatty acids Saturated fatty acids None of the above

  22. Rumen Transformations of Lipid Questions & Answers The Fatty Acid Forum sponsored by

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