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MODUL XII MIKROBIOLOGI TANAH

MODUL XII MIKROBIOLOGI TANAH. Oleh : M. Adhiraga Pratama. Praktikum 6. Siklus nitrogen. 1. amonifikasi. Tujuan Memperlihatkan terlepasnya amoniak dari senyawa organik yang mengandung nitrogen Alat dan bahan Tiga kultur bakteri Tanah subur dan tak subur Reagen nessler

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MODUL XII MIKROBIOLOGI TANAH

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  1. MODUL XIIMIKROBIOLOGI TANAH Oleh : M. AdhiragaPratama

  2. Praktikum 6

  3. Siklus nitrogen

  4. 1. amonifikasi Tujuan • Memperlihatkanterlepasnyaamoniakdarisenyawaorganik yang mengandung nitrogen Alatdanbahan • Tigakulturbakteri • Tanah suburdantaksubur • Reagennessler • Tujuhtabungreaksidenganpepton 4 % • Pembakarbunsen, pipet, jaruminokulasi, spot plate

  5. 1. amonifikasi

  6. 1. amonifikasi

  7. 2. nitrifikasi Tujuan • memperlihatkankonversienzimatikammoniakmenjadinitratolehmikroorganisme Alatdanbahan • Sampeltanahasamdantanahbasa • Duaelenmeyerberisikaldu ammonium sulfatdanduaelenmeyerberisikaldunitrat • Reagennessler, difenilamin, tromsdorf, asamsulfatpekatdanasamsulfatcair • Pembakarbunsen, pipet, spot plate, pengaduk

  8. 2. nitrifikasi

  9. 2. nitrifikasi

  10. 3. denitrifikasi Tujuan • Menunjukanreduksinitratmenjadi gas nitrogen AlatdanBahan • duakulturbakteri • Tanah suburdantaksubur • Empattabungreaksidengantabungdurham • Pembakarbunsen, jaruminokulasi

  11. 3. Denitrifikasi

  12. 4. Fiksasi nitrogen Tujuan • Menunjukanfiksasi nitrogen olehorganismesimbiotikdan non simbiotik AlatdanBahan • Sampeltanahbasa, tanamankacang-kacangansegar • Satuelenmeyerberisimannitoldancawanpetriberisimannitol • Metilenbiru, safranin, kristal violet dan iodine • Bunsen, jaruminokulasi, kacapreparatdanlampu ultraviolet

  13. 4. Fiksasi nitrogen Isolasiazotobacter

  14. 4. Fiksasi Nitrogen Isolasirhizobium

  15. 5. Enumerasi Tujuan • Mengetahui flora dalamtanah • Menentukanjumlahbakteridanjamurdalamtanah Alatdanbahan • Satu gram sampeltanahdanelenmeyerberisi 99 ml air • 4 cawanberisi agar glycerol, 4 cawanberisi agar sabouroud , 4 cawanberisi agar nutrisidan 2 elenmeyerberisi 99 ml air • Bunsen, pipet, colony counter, batanggelas L

  16. 5. Enumerasi 10-3 10-4 10-5 10-6 glycerol 1 99 ml H20 2 99 ml H20 3 99 ml H20 tanah 10-3 10-4 10-5 10-2 sabouroud 10-4 10-5 10-6 10-7 Agar nutrisi

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