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Fungi Laboratory Biology 113. General Laboratory Etiquette. Leave the lab better than you found it! Rinse all glassware. Clean lens of your microscopes and return your microscopes to correct numbered positions. Return prepared slides to the correct slide box
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General Laboratory Etiquette • Leave the lab better than you found it! • Rinse all glassware. • Clean lens of your microscopes and return your microscopes to correct numbered positions. • Return prepared slides to the correct slide box • Wipe down work area with finished with lab.
Lab Study A: Zygomycota • Examine culture of Rhizopus stolonifer using the steromicroscope. • Make a wet mount of Rhizopus and examine with compound microscope. • View prepared slide of Rhizopus stolonifer. • Examine culture of Pilobolus on rabbit dung agar • Study poster.
Rhizopusstolonifer Sporangia Zygosporangia
Lab Study B: Ascomycota • View prepared slide of Claviceps. • View prepared slide of yeast Saccharomyces • Observe plate and do a Wet Mount also • Examine preserved specimen of Peziza. • Examine preserved morels. • Study poster. Also observe plate and do wet mount of Canidida (yeast)
Lab Study C: Basidiomycota • View fresh mushroom under stereoscope. • Examine demonstration specimens of mushrooms, puffballs, shelf fungi. • View prepared slide of Coprinus. • Study poster.
Basidiomycota….Coprinus Coprinus Stalk = Stipe Cap = Pileus Spores form on the surface of the Gills
Lab Study D: Imperfect Fungi (Deuteromycota) • Observe Penicillium culture (compare to Rhizopus) • Observe Aspergillus culture (compare to Rhizopus) • Do a wet mount of a mold to observe live hyphae
Penicillium condiophores with conidia
Lab Stduy E: Lichen • Identify foliose, crustose, and fruticose lichen demonstration materials