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Ming Yan

This project aims to purify and characterize biodegradable polyethylene-degrading enzymes from Streptomyces bacteria and enhance their efficiency through protein engineering techniques.

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Ming Yan

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  1. Ming Yan Purification and Reengineering of Plastic-Degrading Enzymes

  2. Background • Plastic bags are made from polyethylene (PE), a polymer consisting of long chains of the ethylene monomer (C2H4). • Species of bacteria belonging to the Streptomyces genus are capable of producing polyethylene-degrading enzymes.

  3. Streptomyces

  4. Streptomyces • Streptomyces viridosporus • Streptomyces badius • Streptomyces setonii

  5. How is Biodegradability Measured? • Weight loss • Tensile strength loss • measured stress at fracture of the specimen • Changes in percent elongation • extension of material under load • Changes in polyethylene molecular weight distribution • polydispersity

  6. Project Ideas • Aim 1: To purify and characterize the biodegradable polyethylene-degrading enzyme(s) from bacterium belonging to the Streptomyces species • Aim 2: To enhance the biodegradability of the polyethylene by increasing the efficiency of the degrading enzymes through protein engineering techniques

  7. Aim 1 • Inspiration • Enzymes identified for anaerobic degradation of polyethylene glycol (PEG) • Technical Difficulties with the isolation methods

  8. Aim 2 • To enhance the biodegradability of the polyethylene by increasing the efficiency of the degrading enzymes through protein engineering techniques • Methods: • Score system • Protein engineering

  9. Protein-Engineering

  10. More Ideas

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