1 / 9

Dendritic Cell Separation: Techniques and Applications in Immunological Research

Dendritic cells (DCs) are pivotal to the immune system, serving as key antigen-presenting cells that bridge the innate and adaptive immune responses. Their ability to process antigen material and present it on the cell surface to T cells makes them crucial for initiating and modulating immune responses. Understanding and isolating dendritic cells is vital for immunological research and therapeutic development. Advanced cell separation technology, including particle filtration and antibody cell separation, plays a significant role in the efficient isolation and enrichment of dendritic cells for

uberstrainer
Download Presentation

Dendritic Cell Separation: Techniques and Applications in Immunological Research

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. UBERSTRAINER https://uberstrainer.com/

  2. Dendritic Cell Separation: Techniques and Applications in Immunological Research

  3. This article explores the advanced techniques and applications of isolating dendritic cells, highlighting the use of pluriBead technology for efficient and gentle cell separation in immunological research. • Dendritic cells (DCs) are pivotal to the immune system, serving as key antigen-presenting cells that bridge the innate and adaptive immune responses. Their ability to process antigen material and present it on the cell surface to T cells makes them crucial for initiating and modulating immune responses. Understanding and isolating dendritic cells is vital for immunological research and therapeutic development. Advanced cell separation technology, including particle filtration and antibody cell separation, plays a significant role in the efficient isolation and enrichment of dendritic cells for various applications.

  4. pluriBead Technology for Dendritic Cell Isolation • pluriBead offers a unique and efficient cell separation technology that works without any magnetic components. This innovative method involves sieving pluriBeads with bound target cells through a cell strainer. The pluriBeads with the target cells remain on top, while unwanted cells pass through. After detaching the target cells from the beads, researchers have their desired cell population ready for use.

  5. CD11c pluriBead: Specific Isolation of Murine Dendritic Cells • For isolating dendritic cells from murine samples, the CD11c pluriBead suspension is an excellent tool. This product is specifically designed for the positive isolation of CD11c+ target cells from any murine sample material, providing researchers with a reliable method for obtaining high-purity dendritic cells.

  6. Key Features of pluriBead Technology • No Sample Preparation: pluriBead technology simplifies the process by allowing the direct use of samples ranging from 200 µl to 45 ml without the need for density gradient centrifugation or erythrolysis. • Versatile Sample Material: This technology is compatible with various sample materials, including whole blood, buffy coat, PBMC, secretion/excretion material, brain homogenate, spleen, liver, and more. • Fast Isolation: The isolation process can start from as little as 5 minutes, making it highly efficient for researchers with time-sensitive projects.

  7. Gentle Isolation: High yield of viable cells minimizes sample requirement, ensuring that the integrity and functionality of the isolated cells are maintained. • Universal pluriBeads: These beads can be used with any external antibody, allowing for customizable cell separation strategies tailored to specific research needs. • Simultaneous Cell Isolation with pluriBead Cascade: This feature enables the separation of two different cell types at the same time from one sample material, increasing efficiency. • Sequential Cell Isolation: Researchers can isolate up to six different targets from one sample, providing versatility in experimental design.

  8. Conclusion • Dendritic cells are essential for initiating and regulating immune responses, making their isolation and study crucial for advancing immunological research and developing targeted therapies. pluriBead’s advanced cell separation technology, including the CD11c pluriBead for murine samples, offers researchers an efficient and reliable method for isolating high-purity dendritic cells.

  9. Thank you very much! https://uberstrainer.com/

More Related