1 / 17

Aminoacyl tRNA synthetase (aaRS)

Aminoacyl tRNA synthetase (aaRS). Oleh: Ertanti Rizki P. 20512033 Suci Rizki Nurul Aeni 20512044. KK Biokimia-Institut Teknologi Bandung. Outline. T erdapat dalam sel makhluk hidup, yaitu pada Ribosom. What is aa RS ?. Berperan dalam proses translasi RNA.

zody
Download Presentation

Aminoacyl tRNA synthetase (aaRS)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Aminoacyl tRNA synthetase (aaRS) Oleh: Ertanti Rizki P. 20512033 Suci Rizki Nurul Aeni 20512044 KK Biokimia-Institut Teknologi Bandung

  2. Outline

  3. Terdapat dalam sel makhluk hidup, yaitu pada Ribosom What is aa RS ? Berperan dalam proses translasi RNA Pengikatanasam amino spesifikketRNA

  4. Tahapan pengikatantRNAdenganasamamino • Sisiaktifenzimmengikatasam amino dan molekulATP. 2) ATP kehilanganduagugusfosfatdan bergabungdenganasam amino sebagaiAMP. 3) tRNAberikatankovalendenganasam amino menggeserAMP disisiaktifenzim. 4) EnzimmelepaskantRNA-aminoasil, disebut juga“asam amino teraktivasi”

  5. Mekanisme

  6. Perbedaan Kelas Asam Amino

  7. Kinetics

  8. kineticsof Enzyme • Suhu • Konsentrasi enzim • Konsentrasi Substrat • Inhibitor • Jumlah Pyrophosfat

  9. What are development? • Inhibitor enzim ini dapat berguna bagi treatment untuk beberapa penyakit antara lain Malaria dan HIV 1 • Inhibitor enzim ini berpotensi untuk zat antibiotik

  10. Spesific Enzyme Name of IUBMB • EC 6 : Class Ligase • EC 6.1 : Subclass Forming Carbon-Oxygen Bonds • EC 6.1.1 : Ligases forming aminoacyl-tRNA and related compounds • EC 6.1.1.11 : Serine-tRNAligase • Nama lain : seryl-tRNA synthetase; SerRS; seryl-transfer ribonucleate synthetase; seryl-Transfer RNA synthetase; seryl-transfer ribonucleic acid synthetase; serine translase • NamaSistematik: L-serine:tRNASer ligase (AMP-forming)

  11. Function of Serine-tRNAligase • Mengakatalisispengikatankovalendariserinkegugus 3’-OH darinukleotidatRNA-nyadalamsuatuproses yang digerakanolehhidrolisis ATP.

  12. Reaction of Serine-tRNAligase ATP + L-serine + tRNASer AMP + diphosphate + L-seryl-tRNASer

  13. Characteristic of Serine-tRNAligase • Terdapatdua domain yang terikat pada active side dan pada antikodon • Domain N-terminal terdiridarirantai yang sangatpanjang • Seryl-tRNAsynthetasememilikisetidaknya lima spesies yang berbeda yang secarakolektifmengakuienamkodonberbedauntukasam amino

  14. Kineticsof Serine-tRNAligase

  15. Applicationsof Serine-tRNAligase

  16. Modificationsof Serine-tRNAligase

More Related