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Masas spektrometrija

Masas spektrometrija. Jānis Ķimsis jk09021 Rīga, 2014.03.13. Vēsture. 19. gs. beigās Vilhelms Veins atklāj «kanālu staru» noliekšanos elektromagnētiskā lauka ietekmē 20. gs. s ākumā masas spektrometrus izmanto dažādu elementu izotopu atklāšanai

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Masas spektrometrija

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  1. Masas spektrometrija Jānis Ķimsis jk09021 Rīga, 2014.03.13.

  2. Vēsture • 19. gs. beigās Vilhelms Veins atklāj «kanālu staru» noliekšanos elektromagnētiskā lauka ietekmē • 20. gs. sākumā masas spektrometrus izmanto dažādu elementu izotopu atklāšanai • 1940-tajos gados masas spektrometrus sāk izmantot ķīmisko savienojumu analīzē • 1980-tajos gados izgudro MALDI un ESI masas spektrometrijas metodes, to izgudrotājiem 2002 gadā piešķir Nobela balvu ķīmijā https://en.wikipedia.org/wiki/File:Early_Mass_Spectrometer_(replica).jpg https://en.wikipedia.org/wiki/File:Discovery_of_neon_isotopes.JPG

  3. Masas spektrometra uzbūve http://www.ima.umn.edu/2007-2008/MM8.6-15.08/activities/Kearsley-Anthony/fig1.jpg

  4. MALDI un ESI ESI – Electrospray Ionisation MALDI – Matrix Assisted Laser Desorption/Ionization http://www.chm.bris.ac.uk/ms/theory/maldi-ionisation.html http://www.chm.bris.ac.uk/ms/theory/esi-ionisation.html

  5. Proteīnu fingerprintings un sekvenēšana https://upload.wikimedia.org/wikipedia/commons/thumb/1/1f/Mass_spectrometry_protocol.png/625px-Mass_spectrometry_protocol.png

  6. Proteomika Ly et al. 2014

  7. DNS sekvenēšana Murray 1996

  8. Genotipēšana Gassner et al.2013

  9. Secinājumi • Masas spektrometrija nav efektīva DNS sekvenēšanai • Masas spektrometrija ļauj veikt ātru genotipēšanu lielam paraugu daudzumam • Plašs pielietojums -omikās

  10. Izmantotā literatūra • Murray KK, 1996. DNA sequencing by mass spectrometry.J Mass Spectrom. 31(11):1203-15. • Dass C, 2007. Fundamentals of contemporary mass spectrometry. John Wiley & Sons, Inc., 111 River Street, Hoboken, USA. • Gassner C, Meyer S, Frey BM, Vollmert C, 2013. Matrix-assisted laser desorption/ionisation, time-of-flight mass spectrometry-based blood group genotyping--the alternative approach. Transfus Med Rev. 27(1):2-9. • LyT, Ahmad Y, Shlien A, Soroka D, Mills A, Emanuele MJ, Stratton MR, Lamond AI, 2014. A proteomic chronology of gene expression through the cell cycle in human myeloid leukemia cells.

  11. Paldies par uzmanību! Jautājums

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