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Flag-Myc. +. +. +. -. Abl. +. +. -. -. +. STI571. IP:Flag. P-Tyr. Abl. Total:. Myc. -. -. +. +. Abl. -. -. +. +. Abl. N-Myc. -. +. -. +. -. +. -. +. Myc. IP:N-Myc. IP: Myc. P-Tyr. WB:. P-Tyr. WB:. Abl. Abl. Total:. Total:. N-Myc. Myc. b. a. Abl
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Flag-Myc + + + - Abl + + - - + STI571 IP:Flag P-Tyr Abl Total: Myc - - + + Abl - - + + Abl N-Myc - + - + - + - + Myc IP:N-Myc IP: Myc P-Tyr WB: P-Tyr WB: Abl Abl Total: Total: N-Myc Myc b a Abl WT Abl KD Flag-Myc - + - + - - IP:Flag P-Tyr Abl Total: Myc Vinculin c d e Flag-Myc: Y74F + - + - - Flag-Myc Y74F - - - + + Flag-Myc WT - - + - + Abl - - - - + pY74 - - - - + 5YF - - + - + Total WT Flag - - + + + Abl Flag Abl IP: pY74 Abl Total p-Y74 Flag Sanchez-Arévalo et al. Fig. S1