AP Bio: Tuesday, 12.7.10 Intro to PLANTS: Nutrition & Transport

1. AP Bio: Tuesday, 12.7.10Intro to PLANTS: Nutrition & Transport • Homework: • Work on PS 11 (#1, 6, 7) • Do Now: • Take out the Photosynthesis Reading Worksheet • Get back and look over your quiz • Goals for Today: • Describe a structural adaptation and a symbiosis that helps plants’ roots execute their functions • Explain how root anatomy enables plants to selectively uptake water and nutrients • Agenda: • Lecture: Plant Anatomy & Root Adaptations

3. MAJOR PARTS OF A PLANT: • Leaves: • Make sugar through photosynthesis • Release water through transpiration • Stem: • Transports water upwards • Transports sugars wherever needed • Supports/positions leaves • Roots: • Absorb water and nutrients (N, P, K) • Store sugars • Anchor the plant in the soil

4. How do roots absorb water & nutrients? • Structural adaptations • High surface area • Branching structure • Root hairs • Symbioses • Mycorrizalfungi – increase surface area for water and mineral absorption • Rhizobiumbacteria – fix nitrogen (N2) and convert it into a form that plants can use

6. Fungal root symbionts: Mycorrhizae

7. Mycorrhizae

8. Root nodules on legumes:formed by Rhizobium bacteria

9. Example: clover roots

10. How do roots absorb water & nutrients? • Structural adaptations • High surface area • Branching structure • Root hairs • Symbioses • Mycorrizalfungi – increase surface area for water and mineral absorption • Rhizobiumbacteria – fix nitrogen (N2) and convert it into a form that plants can use

11. Cross section of a root:

13. How do water and nutrients enter roots? (First, watch the animation…) • Water moves through the root via two routes: • Symplastic route – Through the cytoplasm & plasmodesmata, after diffusing across the plasma membrane of epidermal cells • Apoplastic route – Along cell walls without entering cells

15. How do water and nutrients enter roots? (First, watch the animation…) • Water moves through the root via two routes: • Symplastic route – Through the cytoplasm & plasmodesmata, after diffusing across the plasma membrane of epidermal cells • Apoplastic route – Along cell walls without entering cells • At the endodermis, the Casparian strip (waxy belt around endodermal cells) forces water and solutes into the cell • Endodermal plasma membrane selects which minerals/nutrients enter • Thereby controlling what enters the xylem • Once inside endodermal cells, water & select solutes flow into xylem cells

17. AP Bio: Wednesday, 12.8.10Water Transport in Plants • Homework: • Work on PS 11 (#2) • Do Now: • Read the article about trees. • On the back, respond to the following : • Summarize the mechanism that pulls water up through plants and trees • What do you remember about water potential? How do you think it relates to water transport in plants? • Goals for Today: • Explain how plants rely on passive mechanisms to transport water from roots to leaves • Design an experiment to measure rates of transpiration in different plants and conditions

18. Water Flow into Roots • Driven by osmosis (sometimes following active transport of minerals into roots) • Due to differences in ψ (water potential) • Quick review: What’s water potential? • Tendency of water to leave an area and go somewhere else • Determined by • Pressure • Solute Concentration • If soil is too dry (ψsoil lower than ψroot cells), water won’t enter roots • Result: wilted plant

21. How do plants transport water up through the xylem? • Xylem structure • Long cells (tracheids & vessel elements) are dead and hollow – just cell walls • Cells connected at ends by holes/perforations for water flow

23. 1. Transpiration creates low WP in leaf air spaces Low WP 2. Water from veins moves into leaves 3. Water in veins is connected by H-bonds all the way down through the xylem. Adhesion & cohesion create a pull on the water below. 4. Water from soil flows in to replace water pulled upwards by TACT High WP

27. How do plants transport water up through the xylem? • Transpiration-Adhesion-Cohesion-Tension Mechanism (TACT) • Transpiration pulls water out of leaves • Water molecules in leaves stick to water molecules all the way down the xylem (cohesion) • Adhesion to cell walls also helps • This creates negative pressure (tension) on the water column in the xylem A completely passive way to transport water, driven by solar energy (powers transpiration) and hydrogen bonds!

28. AP Bio: Wednesday, 12.8.10Water Transport in Plants • Goals for Today: • Explain how plants rely on passive mechanisms to transport water from roots to leaves • Design an experiment to measure rates of transpiration in different plants and conditions

29. Procedure – How to measure transpiration rates • Take the plant out of the pot with the roots and some soil. • Cover the roots/soil with a bag and seal it at the top with a twist-tie. • Measure the initial mass. • Put it in the conditions you’re testing. • Later, measure the mass again (record time between masses). • Calculate % change. • Divide by leaf surface area.

30. Procedure – How to standardize based on surface area Follow the directions on page 6.

31. AP Bio: Thursday, 12.9.10Lab 9, Day 2 – Rates of Transpiration • Homework: • PS 11 due Monday! You will need to research a few questions on your own. • Do Now: • Sit with your lab group • Decide which experiment treatment you want to do! • Goals for Today: • Set up an experiment to measure rates of transpiration • Explain how plants transport sugars through xylem • Agenda: • Lab groups set up experiments & record initial masses • Lecture on sugar transport • Record masses @ end of class

34. Sugar Transport • Phloem • Cells are alive at functional maturity • Two cell types: • Sieve tube elements • Contain only cytoplasm (no nuclei or other organelles) • Connected end to end with sieve holes • Companion cells • Contain the nucleus and organelles to support themselves AND the sieve tube cells

35. How do plants transport sugar from source to sink? • Sugar source – makes or releases sugars • Photosynthesizing leaves • Storage organs that are breaking down/releasing sugar • Sugar sink – absorbs and uses or stores sugars • Actively growing parts • Storage organs that are storing sugar (roots, fruits)

37. SOURCE: Xylem Phloem Sucrose pumped into phloem by active transport. Water follows by osmosis. H2O Sucrose H2O Pulled up by TACT mechanism Positive pressure builds up in phloem near sugar source. Forces sugar-water to flow away from source, towards sink. (Pressure-flow mechanism) Bulk flow due to positive pressure SINK: Sucrose unloaded into sink by active transport. Water diffuses back into xylem to be recycled. Sucrose H2O