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Spektrometrija masa u strukturnoj analizi spojeva: Kako čitati MS spektre?

Spektrometrija masa u strukturnoj analizi spojeva: Kako čitati MS spektre?. Dr. sc. Ivanka Jerić Laboratorij za kemiju ugljikohidrata, peptida i glikopeptida Zavod za organsku kemiju i biokemiju MS info dan – IRB – 6. lipnja 2013. Informacije skrivene u MS spektrima.

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Spektrometrija masa u strukturnoj analizi spojeva: Kako čitati MS spektre?

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  1. Spektrometrija masa u strukturnoj analizi spojeva: Kako čitati MS spektre? Dr. sc. Ivanka Jerić Laboratorij za kemiju ugljikohidrata, peptida i glikopeptida Zavod za organsku kemiju i biokemiju MS info dan – IRB – 6. lipnja 2013.

  2. Informacije skrivene u MS spektrima Kvalitativna analiza• snimanje spektra uzorka• određivanje molekulske mase spoja• analiza fragmentacije određenog molekulskog iona (product ion)• određivanje prekursora određenog fragmenta (precursor ion) Ivanka Jerić - MS info dan

  3. Tekućinska kromatografija Ionizacija Analizator masa Detektor Ivanka Jerić - MS info dan

  4. a Kolizijska ćelija 210 222 268 280 165 170 210 250 290 Full ScanMS (ukupna ionska struja) Q1 Q2 Q3 Ivanka Jerić - MS info dan

  5. a Kolizijska ćelija Product Ion (MS/MS) Q1 Q2 Q3 CID – Collision Induced Dissociation Ivanka Jerić - MS info dan

  6. Peptidi: nomenklatura http://www.ionsource.com/tutorial/DeNovo/DeNovoTOC.htm Ivanka Jerić - MS info dan

  7. Ivanka Jerić - MS info dan

  8. immonium ions Ivanka Jerić - MS info dan

  9. Ivanka Jerić - MS info dan

  10. Primjer: Boc-Phe-Val-OH [M+H]+ = m/z 265 Ivanka Jerić - MS info dan

  11. [M+H]+ = m/z 265 Ivanka Jerić - MS info dan

  12. Primjer: H-Tyr-Pro-Phe-OH [M+H]+ = m/z 426 Ivanka Jerić - MS info dan

  13. [M+H]+ = m/z 426 Ivanka Jerić - MS info dan

  14. Primjer: Boc2-Tyr-Pro-Phe-OGlc [M+H]+ = m/z 788 Ivanka Jerić - MS info dan

  15. Ivanka Jerić - MS info dan

  16. Ivanka Jerić - MS info dan

  17. Ivanka Jerić - MS info dan

  18. Ivanka Jerić - MS info dan

  19. Primjer [M+H]+ = m/z 684 Ivanka Jerić - MS info dan

  20. Ivanka Jerić - MS info dan

  21. [M+H]+ = m/z 764 Ivanka Jerić - MS info dan

  22. Primjer: H-Tyr-Pro-Phe-OGlc Ivanka Jerić - MS info dan

  23. Ivanka Jerić - MS info dan

  24. Za sve dodatne informacije: Ivanka Jerić ivanka.jeric@irb.hr Lidija Brkljačić lidija.brkljacic@irb.hr Journal of Mass Spectrometry American Journal of Mass Spectrometry Rapid Communications in Mass Spectrometry Ivanka Jerić - MS info dan

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