1 / 6

ANALISA ASAM LEMAK DGN GLC

ANALISA ASAM LEMAK DGN GLC. PROF. SBW. PRINSIP ANALISA. MINYAK KACANG/SAMPEL DILARUTKAN DALAM HEKSAN, DITAMBAHKAN LARUTAN KOH METANOLAT, AGAR TERJADI METIL ESTER, CAMPURAN DISENTRIFUS, SUPERNATAN YANG MENGANDUNG METIL ESTER SIAP DIINJEKSIKAN KE GLC. PRINSIP KERJA.

ethan-patton
Download Presentation

ANALISA ASAM LEMAK DGN GLC

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. ANALISA ASAM LEMAK DGN GLC PROF. SBW

  2. PRINSIP ANALISA • MINYAK KACANG/SAMPEL DILARUTKAN DALAM HEKSAN, DITAMBAHKAN LARUTAN KOH METANOLAT, AGAR TERJADI METIL ESTER, CAMPURAN DISENTRIFUS, SUPERNATAN YANG MENGANDUNG METIL ESTER SIAP DIINJEKSIKAN KE GLC.

  3. PRINSIP KERJA • REAGEN: LAR 2 N KOH METANOLAT DAN HEKSAN • TAHAP PERSIAPAN SAMPEL: • TIMBANG MINYAK TEPAT ( 10 mg) larutkandalam 2 ml Heksan, tambahkan 0,2 ml @ M KOH metanolat, Vortex campurantsb, sentrifusdgnkecepatanttt (3000 rpm) selama 5 menit. Supernatanmengandungmetil ester siapdiinjeksikankedalam GLC.

  4. KONDISI MESIN GLC • GLC Hewlett-Packard model 5170 A detektor FID. • Kolom stainless steel 6 ft internal diameter 1/8 inch, diisi 10% DEGS pada 80-100 mesh Chromosorb W/AW • FASE MOBIL: GAS N2 20 ml/menit, H2 20 ml/menit • SUHU INJEKTOR 250 OC, SUHU DETEKTOR 300 OC DAN SUHU KOLOM 195 OC • Sampeldiinjeksikan 1,2 uL • Kfromatogram yang dihasilkansbb:

  5. Kromatogrammetil ester asamlemakminyakkacang

  6. Perhitungankomponenfraksi per sampeldgnlarutanstandarteksternal • PRINSIP PERHITUNGAN: • KADAR STD (STANDART) LUAS AREA STD • KADAR KOMPONEN SAMPEL LUAS AREA KOMPONEN SAMPEL • KADAR KOMPONEN Unknown sampel = • Kadar STD X Luas area Komponen Unknown • Luas area STD

More Related