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Reaksi Enzim

Reaksi Enzim. Reaksi Enzim. Reaksi Enzim. Kinetika Enzim. Leonor Michaelis & Maud Menten. Persamaan Michaelis-Menten :. V 0  Laju / kecepatan awal enzim Vmaks  Laju / Kecepatan maksimum enzim. Km  Konstanta Mechaelis-Menten ( konsentrasi substrat pada saat

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Reaksi Enzim

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  1. ReaksiEnzim

  2. ReaksiEnzim

  3. Reaksi Enzim

  4. KinetikaEnzim Leonor Michaelis & Maud Menten PersamaanMichaelis-Menten: V0 Laju/kecepatanawalenzim Vmaks  Laju/Kecepatanmaksimumenzim Km  KonstantaMechaelis-Menten (konsentrasisubstratpadasaat kecepatansetengahdariVmaks) [S]  KonsentrasiSubstrat

  5. KinetikaEnzim • Bila [S]  Km , maka V berbanding lurusdengan (S) • Bila [S] = Km , maka V = 1/2 Vmax • Bila [S]  Km , maka V = Vmax

  6. Penentuan Km danVmax Plot double reciprocalatau plot Lineweaver-Burk • Plot 1/[S] vs 1/Vo • Persamaan L-B (garislurus/linier) • X-intercept = -1/Km • Y-intercept = 1/Vmax

  7. Penentuan Km danVmax V max Km Km ~ 1.3 mM Vmax ~ 0.25

  8. Penentuan Km danVmax -1/Km = -0.8 Km = 1.23 mM 1/Vmax = 4.0 Vmax = 0.25

  9. Km ??? • Km = [S] pada ½ Vmax • Km affinitasenzimdengansubstrat • 3. Km dapatdigunakanuntukmengevaluasispesifisitasenzimuntukberikatandengansubstrat • Nilai Km kecilartinyaaffinitasenzim-substrattinggi; sebaliknya Km tinggiaffinitasenzim-substratrendah Glucose Km = 8 X 10-6 Allose Km = 8 X 10-3 Mannose Km = 5 X 10-6 HexoseKinase Glucose + ATP <-> Glucose-6-P + ADP

  10. InhibisiEnzim Inhibitor reveribel E + S <-> ES -> E + P E + I <-> EI Ki = [E][I]/[EI] • Competitive • Non-competitive

  11. InhibisiEnzim • Inhibitor mempunyaistruktur yang miripdengansubstrat • Terjadikompetisiantarasubstratdengan inhibitor untukbergabungdengansisiaktifenzim • Efek inhibitor dapatdihilangkandenganpenambahansubstrat • Kompetitif:

  12. Inhibisi Enzim • Peningkatankadarsubstrattdkdapatmeniadakanefek inhibitor • MenurunkanhargaVmax • Berikatandenganenzimpadatempat yang berbedadengantempatpengikatansubstratdanstrukturnyatidakmiripsubstrat • Dapatmengikatenzimbebas (E) maupunkompleksenzim-substrat Non-Kompetitif

  13. Plot Lineweaver-Burk Kompetitif Non-Kompetitif

  14. -COOH H2N- -SONH2 H2N- Aplikasi Inhibitor Para-aminobenzoic acid (PABA) Bakterimemerlukan PABA Untukbiosintesisasamfolat, sehinggabakteridapattumbuh Domagk (1939) • Sulfanilamidmempunyaistruktur yang samadenganPABA, • sehinggabakteritidakdapattumbuh • Sebagai inhibitor enzim • Inhibitor kompetitif Sulfanilamide Sulfa drug (anti-inflammation)

  15. Thank You!

  16. Quiz Gambarkanhubunganantarakecepatanreaksi (aktivitas) terhadapsubstrat (S) Bagaimanacaramenentukannilai Km darigambar(pada no 1) Apaperbedaannyapenentuan Km dengankurva LWB Bandingkan, lebihakurat yang manapenentuan Km (padasoal no 2) dengan (soal no 3) Berikanalasansingkat Berikandefinisinilai Km

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